Central glucagon like peptide-1 inhibits reflex swallowing elicited by the superior laryngeal nerve via caudal brainstem in the rat
Central glucagon like peptide-1 inhibits reflex swallowing elicited by the superior laryngeal nerve via caudal brainstem in the rat
复制标题
中枢胰高血糖素样肽-1抑制大鼠喉上神经通过尾部脑干引起的反射性吞咽
DOI：
10.1016/j.brainres.2017.07.004
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发表时间：
2017
期刊：
影响因子：
2.9
通讯作者：
R.
中科院分区：
文献类型：
--
作者：
Kobashi;M.;Mizutani;S.;Fujita;M.;Mitoh;Y.;Shimatani;Y.;Matsuo;R.
用麻醉大鼠观察胰高血糖素样肽-1（GLP-1）对吞咽反射的影响。使用玻璃微量移液管将GLP-1注射到背侧迷走神经复合体（DVC）中。通过重复电刺激上级喉神经（SLN）的中央切断端诱导吞咽，并通过双极电极穿透下颌舌骨肌的肌电图导线识别吞咽。在内侧DVC（M-DVC）中微量注射GLP-1可增加电刺激SLN时的吞咽频率，并延长首次吞咽的潜伏期。将GLP-1微量注射到外侧DVC（L-DVC）中不会改变吞咽频率或首次吞咽的潜伏期。向M-DVC和L-DVC中注射溶媒均不影响吞咽频率。预先注射GLP-1受体拮抗剂exendin（5-39），除了缩短首次吞咽的潜伏期外，还减弱了GLP-1给药诱导的吞咽频率抑制程度。为了鉴定GLP-1的有效位点，进行了损伤实验。电毁损NTS连合部（cNTS）和去除最后区（AP）的真空并不影响GLP-1注入M-DVC引起的吞咽反射抑制。电损伤NTS内侧核（mNTS）及其附近消除了注射GLP-1引起的吞咽抑制作用。这些结果表明，GLP-1抑制吞咽反射通过背侧延髓中的mNTS。
The effects of glucagon like peptide-1 (GLP-1) on reflex swallowing were examined using anaesthetized rats. GLP-1 was injected into the dorsal vagal complex (DVC) using glass micropipettes. Swallowing was induced by repeated electrical stimulation of the central cut end of the superior laryngeal nerve (SLN) and was identified by the electromyogram lead penetrated in the mylohyoide muscle through bipolar electrodes. Microinjection of GLP-1 into the medial DVC (M-DVC) increased the frequency of swallowing during the electrical stimulation of the SLN and extended the latency of the first swallowing. Microinjection of GLP-1 into the lateral DVC (L-DVC) did not change the frequency of swallowing or the latency of the first swallowing. Neither the injection of vehicle into the M-DVC nor L-DVC affected swallowing frequency. Pre-injection of exendin (5-39), a GLP-1 receptor antagonist, attenuated the degree of suppression of swallowing frequency induced by the administration of GLP-1 in addition to shortening the latency of the first swallowing. To identify the effective site of GLP-1, lesion experiments were performed. Electrical lesion of the commissural part of the NTS (cNTS) and the vacuum removal of the area postrema (AP) did not affect the inhibition of reflex swallowing induced by the injection of GLP-1 into the M-DVC. Electrical lesion of the medial nucleus of the NTS (mNTS) and its vicinity abolished the inhibitory effects of swallowing induced by the injection of GLP-1. These results suggest that GLP-1 inhibits reflex swallowing via the mNTS in the dorsal medulla.