Quantitative fluorescence spectroscopy in turbid media using fluorescence differential path length spectroscopy.
Quantitative fluorescence spectroscopy in turbid media using fluorescence differential path length spectroscopy.
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DOI：
10.1117/1.2992132
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发表时间：
2008-09
影响因子：
3.5
通讯作者：
A. Amelink;B. Kruijt;D. Robinson;H. Sterenborg
中科院分区：
文献类型：
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作者：
A. Amelink;B. Kruijt;D. Robinson;H. Sterenborg
我们开发了一种新技术，荧光差分光程光谱(FDPS)，它能够对混浊介质中的荧光团进行定量研究。FDPS测量采用与差分路径长度光谱(DPS)测量相同的探头几何形状。对两种光纤直径(400微米和800微米)和宽范围的光学特性(Mu(S)：0到10 mm(-1)；Mu(A)：0到2 mm(-1))进行模测量，以研究光学特性对测量的微分荧光信号的影响。对于400微米和800微米纤维，对于给定的荧光团浓度，微分荧光信号在生物相关的散射范围内(0.5至5 mm(-1))分别变化1.4和2.2倍。在路径长度等于差分路径长度的情况下，由于在激发波长处的吸收遵循朗伯-比尔定律，差分荧光信号被衰减。
We have developed a new technique, fluorescence differential path length spectroscopy (FDPS), that enables the quantitative investigation of fluorophores in turbid media. FDPS measurements are made with the same probe geometry as differential path length spectroscopy (DPS) measurements. Phantom measurements are performed for two fiber diameters (400 microm and 800 microm) and for a wide range of optical properties (mu(s)': 0 to 10 mm(-1); mu(a): 0 to 2 mm(-1)) to investigate the influence of the optical properties on the measured differential fluorescence signal. The differential fluorescence signal varies by a factor of 1.4 and 2.2 over the biologically relevant scattering range (0.5 to 5 mm(-1)) for a given fluorophore concentration for 400 microm and 800 microm fibers, respectively. The differential fluorescence signal is attenuated due to absorption at the excitation wavelength following Lambert-Beer's law with a path length equal to the differential path length.