Fluoro-opto-electronic cell-counting on milk
Fluoro-opto-electronic cell-counting on milk
复制标题
牛奶荧光光电细胞计数
DOI：
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发表时间：
1975
影响因子：
2.1
通讯作者：
P. Madsen
中科院分区：
文献类型：
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作者：
P. Madsen
所测设备（Fossomatic）是一种全自动的乳样体细胞计数电子仪器。结果被打印在纸条上并显示在数字计数器上。容量为180个样品/小时。并将仪器的计数结果与直接显微细胞计数和库尔特计数器的颗粒计数结果进行了比较。与显微细胞计数的相关系数为0.922 ~ 0.988，与库尔特计数的相关系数为0.916 ~ 0.992。重现性变异系数为2 - 5%。样品间的结转率为2%。用0.05%重铬酸钾保存牛奶样品不影响细胞计数，而冷冻样品可使细胞计数减少30%。
Summary The equipment tested (Fossomatic) is a fully automatic electronic instrument for counting the somatic cells in milk samples. The results are printed on a paper strip and displayed on a digital counter. The capacity is 180 samples/h. Counting results obtained with the instrument have been compared with direct microscopic cell counts and particle counts by Coulter counter. The correlation coefficient with microscopic cell counts was 0·922–0·988, and with counts by the Coulter counter it was 0·916–0·992. Reproducibility showed a coefficient of variation of 2–5 %. The carry-over between the samples was 2·0 %. Preserving milk samples with 0·05 % potassium dichromate did not influence cell counts, whereas freezing the samples reduced the counts 30 %.