Influence of the protocol used for fibroin extraction on the mechanical properties and fiber sizes of electrospun silk mats
Influence of the protocol used for fibroin extraction on the mechanical properties and fiber sizes of electrospun silk mats
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DOI：
10.1016/j.msec.2013.01.001
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发表时间：
2013-05-01
影响因子：
7.9
通讯作者：
Abel Lozano-Perez, A.
中科院分区：
文献类型：
--
作者：
Aznar-Cervantes, Salvador D.;Vicente-Cervantes, Daniel;Abel Lozano-Perez, A.
丝素蛋白(SF)的再生使用文献中描述的三种最常见的方案(LiBr9.3M，CaCl250wt.%或CaCl2：EtOH：H2O 1：2：8摩尔比)。用SDS-PAGE分析了再生SF在水溶液中的完整性，并观察到不同的降解方案。这一事实也被发现影响冷冻干燥蛋白质的水溶解度。将这些不同的超细纤维用于生产电纺毡，使用SF质量分数为17wt%的SF溶液在1，1，1，1‘，1’，1‘-六氟-2-丙醇(HFIP)中，发现纤维尺寸、伸长率和极限强度值有显著差异。这项工作提供了不同的SF提取方法影响电纺SF垫性能的方式的全局概述，因此应该根据它们的用途来考虑它。(C)2013爱思唯尔B.V.保留所有权利。
Silk fibroin (SF) was regenerated using three of the most common protocols described in the bibliography for the dissolution of raw SF (LiBr 9.3 M, CaCl2 50 wt.% or CaCl2:EtOH:H2O 1:2:8 in molar ratio). The integrity of regenerated SF in aqueous solution was analyzed by SDS-PAGE and different profiles of degradation were observed depending on the protocol used. This fact was found to affect also the aqueous solubility of the freeze dried protein. These different SFs were used to produce electrospun mats using SF solutions of SF 17 wt.% in 1,1,1,1',1',1'-hexafluoro-2-propanol (HFIP) and significant differences in fiber sizes, elongation and ultimate strength values were found. This work provides a global overview of the manner that different methods of SF extraction can affect the properties of electrospun SF-mats and consequently it should be considered depending on the use they are going to be made for. (C) 2013 Elsevier B.V. All rights reserved.