Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern
Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern
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DOI：
10.1371/journal.pone.0172797
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发表时间：
2017-03-06
期刊：
影响因子：
3.7
通讯作者：
Heinemeier, Katja M.
中科院分区：
文献类型：
--
作者：
Eliasson, Pernilla;Svensson, Rene B.;Heinemeier, Katja M.
使用降脂药物他汀类药物治疗在全世界都很常见。最近，自发性肌腱断裂或肌腱病的发生表明他汀类药物对肌腱组织的负面影响。但他汀类药物如何影响肌腱尚不清楚。在本研究中，我们研究了他汀类药物治疗对体外人肌腱样组织的机械强度、细胞增殖、胶原含量和基因表达模式的影响。在3D组织培养模型（肌腱构建物）中培养人肌腱成纤维细胞，并分别用低剂量或高剂量辛伐他汀或阿托伐他汀治疗长达7天。治疗7天后，对构体进行力学测试。测定胶原蛋白含量和细胞增殖。在1天或7天后测量几个目标基因的mRNA水平。7天后，两种他汀类药物均可降低最大力和僵硬度(p
Treatment with lipid-lowering drugs, statins, is common all over the world. Lately, the occurrence of spontaneous tendon ruptures or tendinosis have suggested a negative influence of statins upon tendon tissue. But how statins might influence tendons is not clear. In the present study, we investigated the effect of statin treatment on mechanical strength, cell proliferation, collagen content and gene expression pattern in a tendon-like tissue made from human tenocytes in vitro. Human tendon fibroblasts were grown in a 3D tissue culture model (tendon constructs), and treated with either simvastatin or atorvastatin, low or high dose, respectively, for up to seven days. After seven days of treatment, mechanical testing of the constructs was performed. Collagen content and cell proliferation were also determined. mRNA levels of several target genes were measured after one or seven days. The maximum force and stiffness were reduced by both statins after 7 days (p