Exposure of mice to atrazine and its metabolite diaminochlorotriazine elicits oxidative stress and endocrine disruption
Exposure of mice to atrazine and its metabolite diaminochlorotriazine elicits oxidative stress and endocrine disruption
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小鼠接触莠去津及其代谢物二氨基氯三嗪会引起氧化应激和内分泌干扰
DOI：
10.1016/j.etap.2014.02.014
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发表时间：
2014-03-01
影响因子：
4.3
通讯作者：
Fu, Zhengwei
中科院分区：
文献类型：
--
作者：
Jin, Yuanxiang;Wang, Linggang;Fu, Zhengwei
研究了阿特拉津（ATZ）及其代谢产物二氨基氯三嗪（DACT）对小鼠氧化应激和内分泌干扰的影响。所有ATZ和DACT给药组的体重和肝脏重量均降低。腹腔注射200 mg/kg ATZ、100和200 mg/kg DACT 1周后，肝脏超氧化物歧化酶（SOD）活性显著升高。肝脏过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）的活性也受到200 mg/kg DACT处理的影响。在血清中，谷胱甘肽过氧化物酶（GPX）和GST活性和谷胱甘肽（GSH）含量在200 mg/kg DACT处理组中显著降低。此外，ATZ和DACT给药降低了睾丸中与胆固醇转运和睾酮（T）合成相关的关键基因的转录水平，包括清道夫受体B 1型（SR-B1）、细胞色素P450胆固醇侧链裂解酶（P450 SCC）和细胞色素P450 17 α-羟基类固醇脱氢酶（P450 17 α）。此外，用200 mg/kg DACT处理显著降低血清和睾丸T水平，而用200 mg/kg ATZ处理显著降低睾丸T水平。结果表明，ATZ和DACT急性染毒可引起小鼠氧化应激和内分泌紊乱，且DACT的毒性明显大于ATZ。(C)2014爱思唯尔有限公司版权所有。
Effects of atrazine (ATZ) and its metabolite diaminochlorotriazine (DACT) on the induction of oxidative stress and endocrine disruption were studied in mice. Body and liver weights decreased in all ATZ and DACT treated groups. Hepatic activities of superoxide dismutase (SOD) increased significantly after 1 week of intraperitoneal injection of 200 mg/kg ATZ, 100 and 200 mg/kg DACT. Hepatic activities of catalase (CAT) and glutathione S-transferase (GST) were also affected by the treatment with 200 mg/kg DACT. In serum, the glutathione peroxidase (GPX) and GST activities and glutathione (GSH) content decreased significantly in the 200 mg/kg DACT treated group. Moreover, the administration of ATZ and DACT decreased the transcription levels of key genes related to cholesterol transport and testosterone (T) synthesis including scavenger receptor class B type 1 (SR-B1), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) and cytochrome P450 17 alpha-hydroxysteroid dehydrogenase (P450 17 alpha) in testes. Furthermore, the treatment with 200 mg/kg DACT significantly decreased the serum and testicular T levels, while the treatment with 200 mg/kg ATZ significantly decreased the testicular T levels. The results indicated that the acute exposure to ATZ and DACT induced oxidative stress and endocrine disruption in mice, and DACT showed much more toxic than ATZ did. (C) 2014 Elsevier B.V. All rights reserved.