Tumorigenicity Assays in Nude Mice: Analysis of the Implanted Gelatin Sponge Method
Tumorigenicity Assays in Nude Mice: Analysis of the Implanted Gelatin Sponge Method
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裸鼠致瘤性测定：植入明胶海绵法的分析
DOI：
10.1159/000408620
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
L. Cram
中科院分区：
文献类型：
--
作者：
P. Kraemer;G. Travis;G. Saunders;F. Ray;A. Stevenson;K. Bame;L. Cram
明胶海绵，预植入裸鼠10天，用于培养细胞的致瘤性的改进的测定。通过皮肤接种到这种海绵中的细胞比新植入的海绵或皮下组织更快地产生肿瘤，频率更高。然而，在植入后的最初几天发生了意想不到的细胞损失。这种损失可能是所有类型的致瘤性测定的一个重要方面，并且很容易用所描述的海绵方法进行研究。
Gelatin sponges, preimplanted in nude mice for 10 days, were used for an improved assay for tumorigenicity of cultured cells. Cells inoculated through the skin into such sponges yielded tumors more rapidly and with greater frequency than with newly implanted sponges or into subcutaneous tissue. However, an unexpected loss of cells occurred in the first few days after implantation. This loss may be an important aspect of tumorigenicity assays of all kinds, and is readily studied with the sponge methods described.