Label-free detection of melittin binding to a membrane using electrochemical-localized surface plasmon resonance
Label-free detection of melittin binding to a membrane using electrochemical-localized surface plasmon resonance
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DOI：
10.1021/ac800087u
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发表时间：
2008-03-15
影响因子：
7.4
通讯作者：
Tamiya, Eiichi
中科院分区：
文献类型：
--
作者：
Hiep, Ha Minh;Endo, Tatsuro;Tamiya, Eiichi
局域表面等离子体共振（LSPR）和电化学测量连接到核壳结构的纳米粒子成功地利用在一个同时检测的方案。在这项工作中，这种纳米结构类型的表面等离激元带的特征最初通过控制二氧化硅纳米颗粒的核尺寸和沉积的金的壳厚度来检查。这些结果清楚地表明，当壳厚度增加时，保持核尺寸恒定，LSPR光谱的峰值波长向较短波长移动，并且在特定壳厚度处实现峰值强度的最大值。在此结构的基础上，我们提出了一种基于膜的纳米传感器，用于光学检测肽毒素蜂毒肽与混合双层膜（HBM）的结合，并电化学评估其膜干扰特性作为浓度的函数。它将开辟一条检测功能相似的蛋白质毒素和其他膜靶向肽的途径，目的是将该芯片集成到微流体中并将其扩展为多阵列格式。
Localized surface plasmon resonance (LSPR) and electrochemistry measurements connecting to core-shell structure nanoparticle are successfully exploited in a simultaneous detectable scheme. In this work, the surface plasmon band characterizations of this nanostructure type are initially examined by controlling the core size of the silica nanoparticle and shell thickness of the deposited gold. These results clearly show that when the shell thickness is increased, keeping the core size constant, the peak wavelength of the LSPR spectra is shifted to a shorter wavelength and the maximum of peak intensity is achieved at a particular shell thickness. On the,basis of this structure, we present a membrane-based nanosensor for optically detecting the binding of peptide toxin melittin to hybrid bilayer membrane (HBM) and electrochemically assessing its membrane-disturbing properties as a function of concentrations. It will open up the way to detect functionally similar protein toxins and other membrane-targeting peptides with the intension of integrating this chip into a microfluid and expanding it into multiarray format.