Occurrence of saxitoxins as a major toxin in the ovary of a marine puffer Arothron firmamentum
Occurrence of saxitoxins as a major toxin in the ovary of a marine puffer Arothron firmamentum
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DOI：
10.1016/j.toxicon.2003.05.001
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发表时间：
2004-02-01
期刊：
影响因子：
2.8
通讯作者：
Noguchi, T
中科院分区：
文献类型：
--
作者：
Nakashima, K;Arakawa, O;Noguchi, T
采自日本大分县和岩手县的硬脊叉鼻鲀（Arothron firmamentum），雌雄各11只。用小白鼠进行的毒性试验表明，仅雌性样品的卵巢和皮肤有毒性，毒性评分分别为5-740（麻痹性贝毒）和< 5-30 MU/g（河豚毒素）。毒素提取物从这两个组织，然后处理与筒柱，并进行高效液相色谱和液相色谱-质谱分析。在分析中，石房蛤毒素（STX）和decarbamoyISTX（dcSTX）被确定为卵巢中的主要毒素，而皮肤仅含有TTX（C）2003爱思唯尔有限公司保留所有权利。
Eleven male and 14 female specimens of a marine puffer Arothron firmamentum were collected from Oita and Iwate Prefectures, Japan. The toxicity assay using mouse showed that only ovary and skin of the female specimens were toxic, the toxicity scores being 5-740 as paralytic shellfish poison and < 5-30 MU/g as tetrodotoxin (TTX), respectively. The toxin extracts from the both tissues were then treated with cartridge columns, and subjected to high performance liquid chromatography and liquid chromatography-mass spectral analyses. In the analyses, saxitoxin (STX) and decarbamoyISTX (dcSTX) were identified as the major toxins in the ovary, while the skin contained only TTX (C) 2003 Elsevier Ltd. All rights reserved.