Repair of potentially mutagenic damage, sensitive to anisotonic treatment, in actively growing Chinese hamster cells.
Repair of potentially mutagenic damage, sensitive to anisotonic treatment, in actively growing Chinese hamster cells.
复制标题
修复活跃生长的中国仓鼠细胞中对异渗处理敏感的潜在诱变损伤。
DOI：
10.1080/09553008814551661
复制
发表时间：
1988
影响因子：
2.6
通讯作者：
Hill,CK
中科院分区：
文献类型：
--
作者：
Kubota,N;Hill,CK
利用指数生长的中国仓鼠V79细胞，研究了磷酸盐缓冲盐水(PBS)处理对X射线照射后突变频率的影响。对次黄嘌呤-鸟嘌呤磷酸核糖转移酶基因(HGPRT)进行了突变诱导研究。当细胞受到X射线照射后，立即用0.5mNaC l/PBS在37℃下处理20min，细胞存活率显著下降，突变体数量也随之增加，这表明辐射后用非等渗盐溶液处理细胞时，潜在的致死损伤和潜在的突变损伤分别转化为致死损伤和诱变损伤。0.5mNaC l/PBS对5.86GyX射线照射后的潜在致突变损伤的修复动力学与潜在致死损伤的修复动力学相似。两种损伤的修复在X射线照射后40分钟内基本完成。
Using exponentially growing Chinese hamster V79 cells we studied the effect of anisotonic phosphate-buffered saline (PBS) treatment on the frequency of mutation following X-irradiation. Induction of mutants was studied at the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT). When cells were X-irradiated and immediately followed by 0·5mNaCl/PBS treatment for 20 min at 37°C, cell survival was significantly decreased and a concomitant increase in the number of mutants was observed, indicating that postirradiation treatment with anisotonic salt solution caused conversions of potentially lethal damage and potentially mutagenic damage to lethal and mutagenic damage, respectively. The repair kinetics of potentially mutagenic damage expressible by 0·5mNaCl/PBS after 5·86 Gy of X-rays were similar to those of potentially lethal damage. The repair of both types of damage was essentially complete within 40 min after X-irradiation.