Systemic and cerebrospinal fluid immune and complement activation in Ugandan children and adolescents with long-standing nodding syndrome: A case-control study.
Systemic and cerebrospinal fluid immune and complement activation in Ugandan children and adolescents with long-standing nodding syndrome: A case-control study.
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DOI：
10.1002/epi4.12463
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发表时间：
2021-06
期刊：
影响因子：
3
通讯作者：
Idro R
中科院分区：
文献类型：
--
作者：
Ogwang R;Muhanguzi D;Mwikali K;Anguzu R;Kubofcik J;Nutman TB;Taylor M;Newton CR;Vincent A;Conroy AL;Marsh K;Idro R
Nodding syndrome is a poorly understood epileptic encephalopathy characterized by a unique seizure type—head nodding—and associated with Onchocerca volvulus infection. We hypothesized that altered immune activation in the cerebrospinal fluid (CSF) and plasma of children with nodding syndrome may yield insights into the pathophysiology and progression of this seizure disorder. We conducted a case‐control study of 154 children (8 years or older) with long‐standing nodding syndrome and 154 healthy age‐matched community controls in 3 districts of northern Uganda affected by nodding syndrome. Control CSF samples were obtained from Ugandan children in remission from hematological malignancy during routine follow‐up. Markers of immune activation and inflammation (cytokines and chemokines) and complement activation (C5a) were measured in plasma and CSF using ELISA or Multiplex Luminex assays. O volvulus infection was assessed by serology for anti–OV‐16 IgG levels. The mean (SD) age of the population was 15.1 (SD: 1.9) years, and the mean duration of nodding syndrome from diagnosis to enrollment was 8.3 (SD: 2.7) years. The majority with nodding syndrome had been exposed to O volvulus (147/154 (95.4%)) compared with community children (86/154 (55.8%)), with an OR of 17.04 (95% CI: 7.33, 45.58), P < .001. C5a was elevated in CSF of children with nodding syndrome compared to controls (P < .0001). The levels of other CSF markers tested were comparable between cases and controls after adjusting for multiple comparisons. Children with nodding syndrome had lower plasma levels of IL‐10, APRIL, CCL5 (RANTES), CCL2, CXCL13, and MMP‐9 compared with community controls (P < .05 for all; multiple comparisons). Plasma CRP was elevated in children with nodding syndrome compared to community children and correlated with disease severity. Nodding syndrome is associated with exposure to O. volvulus. Compared to controls, children with long‐standing symptoms of nodding syndrome show evidence of complement activation in CSF and altered immune activation in plasma.
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影响因子：
2.9
作者：
Idro R;Opoka RO;Aanyu HT;Kakooza-Mwesige A;Piloya-Were T;Namusoke H;Musoke SB;Nalugya J;Bangirana P;Mwaka AD;White S;Chong K;Atai-Omoruto AD;Mworozi E;Nankunda J;Kiguli S;Aceng JR;Tumwine JK
通讯作者：
Tumwine JK
影响因子：
3.8
作者：
Levick, Bethany;Laudisoit, Anne;Colebunders, Robert
通讯作者：
Colebunders, Robert
影响因子：
1
作者：
Idro, R.;Musubire, K. A.;Mbonye, A. K.
通讯作者：
Mbonye, A. K.
影响因子：
2.9
作者：
Idro R;Namusoke H;Abbo C;Mutamba BB;Kakooza-Mwesige A;Opoka RO;Musubire AK;Mwaka AD;Opar BT
通讯作者：
Opar BT
影响因子：
3.7
作者：
Foltz JL;Makumbi I;Sejvar JJ;Malimbo M;Ndyomugyenyi R;Atai-Omoruto AD;Alexander LN;Abang B;Melstrom P;Kakooza AM;Olara D;Downing RG;Nutman TB;Dowell SF;Lwamafa DK
通讯作者：
Lwamafa DK