The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans.
The WOPR Domain Protein OsaA Orchestrates Development in Aspergillus nidulans.
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DOI：
10.1371/journal.pone.0137554
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yu JH
中科院分区：
文献类型：
--
作者：
Alkahyyat F;Ni M;Kim SC;Yu JH
在细粒曲霉的生命周期中发生细胞生长发育的协调过程。一项旨在揭示新的发育调控因子的多拷贝遗传筛选发现了AN6578位点，该位点预计编码一个具有WOPR结构域的蛋白质，这是一个广泛存在的真菌特异性dna结合基序。AN6578的多拷贝破坏了真菌的正常生命周期，导致营养细胞的增殖增强，而缺失则导致性结实过度活跃，无性发育（分生）减少，因此被命名为osaA （Orchestrator of Sex and Asex）。进一步的遗传研究表明，OsaA主要通过抑制天鹅绒调节因子VeA下游的性发育来平衡发育。缺乏osaA足以抑制veA1等位基因，导致产孢水平与veA +野生型（WT）相当。通过RNA-Seq对WT、veA1和ΔosaA veA1菌株进行全基因组转录组学分析，进一步证实了OsaA在VeA下游具有抑制性发育的功能。然而，OsaA在控制发育中也起着额外的作用，因为与WT相比，ΔosaA veA1突变体表现出早熟和增强的h<s:1> lle细胞形成。黄曲霉的OsaA同源物能够补充a . nidulans中的OsaA null表型，表明这组WOPR结构域蛋白的保守作用。综上所述，OsaA是曲霉形态和化学发育的上游协调者，在VeA下游发挥作用。
Orchestration of cellular growth and development occurs during the life cycle of Aspergillus nidulans. A multi-copy genetic screen intended to unveil novel regulators of development identified the AN6578 locus predicted to encode a protein with the WOPR domain, which is a broadly present fungi-specific DNA-binding motif. Multi-copy of AN6578 disrupted the normal life cycle of the fungus leading to enhanced proliferation of vegetative cells, whereas the deletion resulted in hyper-active sexual fruiting with reduced asexual development (conidiation), thus named as osaA (Orchestrator of Sex and Asex). Further genetic studies indicate that OsaA balances development mainly by repressing sexual development downstream of the velvet regulator VeA. The absence of osaA is sufficient to suppress the veA1 allele leading to the sporulation levels comparable to veA + wild type (WT). Genome-wide transcriptomic analyses of WT, veA1, and ΔosaA veA1 strains by RNA-Seq further corroborate that OsaA functions in repressing sexual development downstream of VeA. However, OsaA also plays additional roles in controlling development, as the ΔosaA veA1 mutant exhibits precocious and enhanced formation of Hülle cells compared to WT. The OsaA orthologue of Aspergillus flavus is able to complement the osaA null phenotype in A. nidulans, suggesting a conserved role of this group of WOPR domain proteins. In summary, OsaA is an upstream orchestrator of morphological and chemical development in Aspergillus that functions downstream of VeA.