Abnormal estrous cyclicity and behavioral hyporesponsiveness to ovarian hormones in genetically obese Zucker female rats
Abnormal estrous cyclicity and behavioral hyporesponsiveness to ovarian hormones in genetically obese Zucker female rats
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DOI：
10.1210/en.138.1.143
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发表时间：
1997-01-01
期刊：
影响因子：
4.8
通讯作者：
Olster, DH
中科院分区：
文献类型：
--
作者：
Bivens, CLM;Olster, DH
肥胖的扎克母鼠不育。本研究旨在评估成人，卵巢完整，精益ana的发情周期。并比较外源性类固醇激素对去卵巢（ovx）瘦和肥胖Zucker大鼠的生殖行为的影响。大多数（90%）肥胖大鼠的周期不完整，而瘦弱的Zucker大鼠的周期为1天。切除卵巢后，各组大鼠分别给予苯甲酸雌二醇（EB, 15 ~ 100 μ g/kg）或EB +黄体酮（P, 2 ~ 20 mg/kg）治疗，检测性接受性和性感受性（PRO）。在最高的EB剂量下，肥胖的雌性Zucker出现前凸的频率低于瘦鼠（前凸商数LQ分别为8 +/- 68和32 +/- 138）。在最低剂量的EB + P下，瘦雌鱼的接受性和感知性极好（LQ = 93 +/- 4%, PRO = 6.2 +/- 2次/分钟）。相比之下，Zucker肥胖的雌性只有轻微的接受性（LQ = 26 +/- 11%）， PRO低于瘦大鼠（PRO = 2.4 +/- 0.6次/分钟）。增加EB或P的剂量，并结合最低剂量的另一种激素，在肥胖的雌性朱克身上产生了接受性和PRO，这与在瘦老鼠身上观察到的结果相当。当给予相同剂量的激素时，卵巢肥胖大鼠的血清雌二醇和P水平与卵巢瘦大鼠相当或更高。这些数据表明，与瘦肉大鼠相比，肥胖的Zucker需要更高的EB和/或P的剂量和血清浓度才能引起强健的前凸和PRO。这种对性类固醇激素反应低下的行为可能导致肥胖的Zucker雌性大鼠不孕。
Obese Zucker female rats are infertile. The present study was designed to assess estrous cyclicity in adult, ovary-intact, lean ana. obese Zucker rats and to compare reproductive behaviors induced by exogenous steroid hormones in ovariectomized (ovx) lean and obese Zucker rats. The majority (90%) of obese rats had incomplete cycles in comparison with the normal, l-day cycles displayed by lean Zucker rats. After ovariectomy, all rats were treated with estradiol benzoate (EB, 15-100 mu g/kg) or EB plus progesterone (P, 2-20 mg/kg), and tested for sexual receptivity and proceptivity (PRO). At the highest EB dose, obese Zucker females displayed lordosis less frequently than lean rats (lordosis quotient, LQ, 8 +/- 68 vs. 32 +/- 138, respectively). At the lowest doses of EB plus P, lean females were extremely receptive and preceptive (LQ = 93 +/- 4%, PRO = 6.2 +/- 2 bouts/min). Zucker obese females, in contrast, were only slightly receptive (LQ = 26 +/- 11%) and showed less PRO than lean rats (PRO = 2.4 +/- 0.6 bouts/min). Increasing the dose of either EB or P, administered in combination with the lowest dose of the other hormone, produced receptivity and PRO in obese Zucker females that were comparable with those observed in lean rats. Serum estradiol and P levels in ovx obese rats were either equivalent to or higher than those in the ovx lean rats when both were given the same doses of hormones. These data suggest that considerably higher doses and serum concentrations of EB and/or P are required to elicit robust lordosis and PRO in ovx obese Zucker than in lean rats. This behavioral hyporesponsiveness to sex steroid hormones may contribute to infertility in the obese Zucker female rat.