Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations
Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations
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DOI：
10.1016/j.jmoldx.2018.03.004
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发表时间：
2018-07-01
影响因子：
4.1
通讯作者：
Kramer, Fred R.
中科院分区：
文献类型：
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作者：
Vargas, Diana Y.;Marras, Salvatore A. E.;Kramer, Fred R.
在设计用于检测罕见体细胞突变的PCR测定中，SuperSelective引物凭借其短的3 '-足序列，选择性地启动突变DNA靶片段的合成，同时抑制相关野生型片段的合成，并且所得阈值循环反映存在的突变靶的量。但如果系统仍有
In PCR assays designed to detect rare somatic mutations, SuperSelective primers, by virtue of their short 3'-foot sequences, selectively initiate synthesis on mutant DNA target fragments, while suppressing the synthesis of related wild-type fragments, and the resulting threshold cycle reflects the quantity of mutant targets present. However, when there are