Synthesis of long-chain polyunsaturated fatty acids in preterm newborns fed formula with long-chain polyunsaturated fatty acids
Synthesis of long-chain polyunsaturated fatty acids in preterm newborns fed formula with long-chain polyunsaturated fatty acids
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DOI：
10.1093/ajcn/86.5.1323
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发表时间：
2007-11-01
影响因子：
7.1
通讯作者：
Cogo, Paola E.
中科院分区：
文献类型：
--
作者：
Carnielli, Virgilio P.;Simonato, Manuela;Cogo, Paola E.
背景资料：二十二碳六烯酸（DHA）和花生四烯酸（AA）是长链多不饱和脂肪酸（LCPs），在生长发育和神经发育中起着重要作用。目的：定量测定早产儿配方奶粉中LCPs的合成。设计图：22名早产儿被随机分配到无LCP组（喂食不含LCP的配方奶粉; n = 11）或LCP组（喂食含LCP的配方奶粉; n = 11）。膳食中的LCPs比内源合成的LCPs（由亚油酸和α-亚麻酸衍生）具有更高的C-13含量。采用气相色谱-同位素比质谱法测定出生时和1、3、7月龄时主要选定血浆脂肪酸的C-13含量。计算绝对LCP合成和LCP合成相对于膳食摄入量的百分比。在1，3，和7月龄。在1、3和7个月时，AA的绝对合成量分别为26.7 +/-4.2、14.4 +/- 3.9和11.6 +/- 4.1 mg中心点kg（-1）d（-1），DHA的绝对合成量分别为12.6 +/- 4.5、3.2 +/- 2.5和2.3 +/- 1.9 mg中心点kg（-1）d（-1）。分别AA和DHA的合成随时间显著下降（P < 0.01），AA的合成显著（P < 0.01）大于DHA的合成。结论：通过这种新的方法，我们测量了内源性LCP合成婴儿接受饮食LCP长时间。到7个月时，喂养LCP的早产儿的LCP合成显著降低。
Background: Docosahexaenoic acid (DHA) and arachidonic acid (AA) are long-chain polyunsaturated fatty acids (LCPs) that play pivotal roles in growth and neurodevelopment.Objective: We aimed to quantify the synthesis of LCPs in preterm infants fed infant formula containing LCPs. Design: Twenty-two preterm infants were randomly assigned to either the no-LCP group (fed formula without LCPs; n = 11) or the LCP group (fed formula with LCPs; n = 11). Dietary LCPs had higher C-13 content than did the endogenously synthesized LCPs which were derived from linoleic and a-linolenic acids. The C-13 content of major selected plasma fatty acids was measured by using gas chromatography-isotope ratio mass spectrometry at birth and at age 1, 3, and 7 mo. Absolute LCP synthesis and the percentage of LCP synthesis relative to dietary intake were calculated.Results: Percentage AA synthesis was 67.2 +/- 7.8%, 35.9 +/- 9.8%, and 29.0 +/- 10.3%, and that of DHA was 41.7 +/- 14.9%,10.5 +/- 8.1%, and 7.4 +/- 6.2% at 1, 3, and 7 mo old, respectively. Absolute AA synthesis was 26.7 +/- 4.2,14.4 +/- 3.9, and 11.6 +/- 4.1 mg center dot kg(-1)d(-1) and that of DHA was 12.6 +/- 4.5, 3.2 +/- 2.5, and 2.3 +/- 1.9 mg center dot kg(-1) center dot d(-1) at 1, 3, and 7 mo old, respectively. AA and DHA synthesis decreased significantly (P < 0.01) with time, and AA synthesis was significantly (P < 0.01) greater than DHA synthesis.Conclusions: By this novel approach, we measured endogenous LCP synthesis in infants receiving dietary LCPs over long periods. By age 7 mo, LCP synthesis was dramatically lower in preterm infants fed LCPs.