Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
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Tn5 诱导的 2,5-二氯氢醌降解缺陷鞘氨醇单胞菌突变体的分离和表征
DOI：
10.1271/bbb.60.689
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
M. Takagi
中科院分区：
文献类型：
--
作者：
Y. Nagata;K. Miyauchi;Seug;A. Futamura;M. Takagi
少见鞘氨醇单胞菌UT26使用六六六(γ-γ)作为碳和能源的唯一来源。在UT26中，γ-六六六被转化为2，5-二氯对苯二酚(2，5-DCHQ)。为了克隆与2，5-二氯甲烷降解相关的基因，将Tn5突变引入到UT26中，并通过喷洒γ-六氯环己烷溶液的方法对3株2，5-二氯甲烷降解缺陷突变株(UT102、UT103和UT116)进行了可视化筛选。利用静息细胞分析了这3个突变体和UT26对2，5-DCHQ和对苯二酚(HQ)的降解活性。这三个突变体具有不同的表型。2，5-CHQ可诱导UT26细胞表达2，5-DCHQ和HQ降解活性。
Sphingomonas paucimobilis UT26 uses γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In UT26, γ-HCH is converted to 2,5-dichlorohydroquinone (2,5-DCHQ). To clone a gene that is involved in the degradation of 2,5-DCHQ, a Tn5 mutation was introduced into UT26, and three mutants (UT102, UT103, and UT116) defective in 2,5-DCHQ degradation were visually selected by spraying γ-HCH solution on the colonies. 2,5-DCHQ and hydroquinone (HQ) degradation activities of these three mutants and UT26 were analyzed using resting cells. The three mutants had different phenotypes. It was also demonstrated that 2,5-DCHQ and HQ degradation activities were inducibly expressed in UT26 in the presence of 2,5-CHQ.