New-type inducers of differentiation of HL-60 leukemia cells suppress c-myc expression.
New-type inducers of differentiation of HL-60 leukemia cells suppress c-myc expression.
复制标题
新型HL-60白血病细胞分化诱导剂抑制c-myc表达。
DOI：
--
复制
发表时间：
1987
影响因子：
1.7
通讯作者：
K. Shudo
中科院分区：
文献类型：
--
作者：
Y. Hashimoto;H. Kagechika;E. Kawachi;K. Shudo
新型诱导人早幼粒细胞白血病细胞HL-60分化的诱导剂4-（5,6,7,8 -四氢- 5,5,8,8 -四甲基-2-萘基氨基甲酰）苯甲酸（am80）和(E) -4-[3-（3,5 -二丁基苯基）-3-氧-l-丙烯基]苯甲酸（ch55）与全反式维甲酸（RA）一样抑制c-myc的表达。c-myc表达水平在处理开始后5-14 h内检测到，并且在形态分化和功能分化之前以硝基蓝四氮唑（NBT）减少来测量。用0.1 μm Am 80、Ch 55或RA处理HL-60细胞96 h，可抑制90%以上的c-myc表达，nbt阳性细胞比例增加约10倍。
New-type inducers of differentiation of human promyelocytic leukemia cells HL-60, i.e., 4- (5, 6, 7, 8-tetrahydro-5, 5, 8, 8-tetramethyl-2-naphthalenylcarbamoyl) benzoic acid (Am 80) and (E) -4- [3- (3, 5-di-tert-butylphenyl) -3-oxo-l-propenyl] benzoic acid (Ch 55), suppress c-myc expression as all-trans-retinoic acid (RA) does. The decreased level of c-myc expression was detected within 5-14 h after the start of treatment, and preceded morphological differentiation and functional differentiation measured in terms of nitroblue tetrazolium (NBT) reduction. Treatment of HL-60 cells with 0.1 μm Am 80, Ch 55 or RA for 96 h caused more than 90% suppression of c-myc expression and around 10-fold increase in the percentage of NBT-positive cells.