Genetic variation in wild and cultured populations of the pearl oyster Pinctada fucata from southern China
Genetic variation in wild and cultured populations of the pearl oyster Pinctada fucata from southern China
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DOI：
10.1016/j.aquaculture.2006.03.024
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发表时间：
2006-08
期刊：
影响因子：
4.5
通讯作者：
Dahui Yu;K. Chu
中科院分区：
文献类型：
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作者：
Dahui Yu;K. Chu
为给我国合浦珠母贝的选育提供依据，采用AFLP分子标记技术对中国南方合浦珠母贝野生和养殖群体的遗传变异进行了研究。3对引物在北部湾、大亚湾和三亚湾的179个个体中共扩增出182个位点。结果表明，野生群体和养殖群体的遗传多样性均较高，变异系数从三亚湾野生群体的0.367到北部湾野生群体的0.393，多态位点比例较高。但三亚湾和北部湾养殖群体比相应的野生群体具有更多的固定位点。大多数群体的两两比较中，遗传分化是显著的。AMOVA分析表明，居群间的遗传变异虽然显著，但很低（1.77%），而98%以上的变异存在于居群内的个体间。这些结果没有证据表明中国珠母贝的孵化实践对养殖群体的遗传多样性产生了显著影响，表明所有群体都具有选择能力。然而，大多数群体之间显着的遗传分化意味着任何易位的个人遗传改良计划应谨慎管理，以保持自然群体的遗传多样性。
In order to provide information for selective breeding program in China, the genetic variation in wild and cultured Pinctada fucata populations from southern China was studied using AFLP markers. Three pairs of primers generated 182 loci among 179 individuals in populations from Beibu Bay, Daya Bay and Sanya Bay. A high level of genetic diversity, ranging from 0.367 in a wild population in Sanya Bay to 0.393 in a wild population in Beibu Bay, and high level of proportion of polymorphic loci were observed within both wild and cultured populations. Yet cultured populations in Sanya Bay and Beibu Bay had more fixed loci than the corresponding wild populations. Genetic differentiation in most pairwise comparisons of populations was significant. AMOVA indicated that genetic variation among populations was very low (1.77%) though significant, while more than 98% variation resided among individuals within population. These findings provide no evidence to show that hatchery practice of pearl oyster in China to date has significantly affected the genetic diversity of the cultured populations, and suggest that all populations are competent for selection. Yet the significant genetic differentiation among most populations implies that any translocation of individuals for genetic improvement program should be managed with caution for the preservation of genetic diversity in natural populations.