Handheld readout system for field‐effect transistor biosensor arrays for label‐free detection of biomolecules
Handheld readout system for field‐effect transistor biosensor arrays for label‐free detection of biomolecules
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DOI：
10.1002/pssa.201431862
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发表时间：
2015-06
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影响因子：
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通讯作者：
T. C. Nguyen;M. Schwartz;X. Vu;J. Blinn;S. Ingebrandt
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文献类型：
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作者：
T. C. Nguyen;M. Schwartz;X. Vu;J. Blinn;S. Ingebrandt
使用离子敏感场效应晶体管（ISFET）阵列在液体环境中工作的无标记生物分子检测传感器为疾病的快速诊断和其他护理点应用提供了巨大的机会。在过去的几十年中，描述了基于ISFET的传感器，其具有逐渐提高的灵敏度、鲁棒性和检测生物分子的多样性。然而，一个可靠的，小型化的电子读出这样的传感器是在该领域的主要需求之一。在这项工作中，我们开发了一种手持式读出系统，用于ISFET阵列的生物分子传感，该系统可以同时测量四个通道，并可用于从微尺寸ISFET到硅纳米线晶体管阵列的各种ISFET器件。硬件是围绕32位PIC微控制器构建的。一个用LabVIEW编写的用户友好型软件与硬件进行通信，以记录和显示测量结果。经过简单的校准，手持系统与标准半导体参数分析仪相比，误差小于5%。对于第一个应用，pH传感和脱氧核糖核酸（DNA）的固定和杂交事件成功地测量与我们的ISFET传感器。我们的读出系统与自上而下制造的传感器器件相结合，有可能在不久的将来使ISFET传感器更接近床旁检测。
Sensors for label‐free biomolecule detection using ion‐sensitive field‐effect transistor (ISFET) arrays working in a liquid environment offer great opportunities for fast diagnostics of diseases and other point‐of‐care applications. In the last decades, ISFET‐based sensors with progressively improved sensitivity, robustness, and diversity of detected biomolecules were described. However, a reliable, miniaturized electronic readout for such sensors is one of the main demands in the field. In this work, we developed a handheld readout system for biomolecule sensing with ISFET arrays, which can measure four channels simultaneously and can be used for a wide range of ISFET devices from microsized ISFETs to silicon nanowire transistor arrays. The hardware was built around a 32‐bit PIC microcontroller. A user‐friendly software written in LabVIEW communicates with the hardware to record and to display the measurement results. After a simple calibration, the error of the handheld system was below 5% in comparison with a standard semiconductor parameter analyzer. For the first applications, pH sensing and deoxyribonucleic acid (DNA) immobilization and hybridization events were successfully measured with our ISFET sensors. Our readout system in combination with our top‐down fabricated sensor devices has the potential to bring the ISFET sensors closer to the point‐of‐care testing in near future.