The plant sterol brassicasterol as additional CSF biomarker in Alzheimer's disease
The plant sterol brassicasterol as additional CSF biomarker in Alzheimer's disease
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DOI：
10.1111/j.1600-0447.2011.01713.x
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发表时间：
2011-09-01
影响因子：
6.7
通讯作者：
Luetjohann, D.
中科院分区：
文献类型：
--
作者：
Vanmierlo, T.;Popp, J.;Luetjohann, D.
目的：植物甾醇（谷甾醇、菜油甾醇、豆甾醇和菜子甾醇）仅是结构上与胆固醇非常相似的膳食衍生甾醇。与外周胆固醇相反，植物甾醇可以穿过血脑屏障并在哺乳动物脑内积累。作为一个受损的功能的脑脊液（CSF）-血液屏障与神经退行性疾病，即阿尔茨海默病（AD），我们调查了这是否会导致改变植物甾醇浓度在CSF.Method：应用气相色谱/质谱分析，植物甾醇浓度测定血浆和CSF中的患者AD（n = 67）和对照组（n = 29）。年龄，性别，血浆-CSF白蛋白比，CSF A β（42），CSF pTau，APOE 4基因型，和血清肌酐作为协变量的统计分析，个别植物甾醇，以比较血浆和CSF中的植物甾醇浓度AD患者和controls.Results之间：白蛋白商是一个一致的预测在CSF中胆固醇和甲基植物甾醇菜油甾醇和菜籽甾醇。基于相关预测因子的每个诊断的脂质参数的比较显示AD患者CSF中的油菜甾醇浓度显著较低（P < 0.001）。二元逻辑回归分析显示，在生物标志物模型中，当添加到pTau和A β（42）中时，油菜甾醇提高了预测值。
Objective: Plant sterols (sitosterol, campesterol, stigmasterol and brassicasterol) are solely dietary-derivable sterols that are structurally very similar to cholesterol. In contrast to peripheral cholesterol, plant sterols can cross the blood-brain barrier and accumulate within mammalian brain. As an impaired function of the cerebrospinal fluid (CSF)-blood barrier is linked to neurodegenerative disorders, i.e. Alzheimer's disease (AD), we investigated whether this results in altered plant sterol concentrations in CSF.Method: Applying gas chromatography/mass spectrometry analysis, plant sterol concentrations were measured in plasma and CSF of patients with AD (n = 67) and controls (n = 29). Age, gender, plasma-to-CSF albumin ratio, CSF A beta(42), CSF pTau, APOE4 genotype, and serum creatinine were applied as covariates in the statistical analysis for individual plant sterols in order to compare plasma and CSF plant sterol concentrations between patients with AD and controls.Results: Albumin quotient was a consistent predictor in CSF for cholesterol and methyl plant sterols campesterol and brassicasterol. Comparison of lipid parameters per diagnosis based on relevant predictors revealed significantly lower concentrations of brassicasterol (P < 0.001) in CSF of patients with AD. Binary logistic regression analysis revealed that brassicasterol improved the predictive value when added to pTau and A beta(42) in a biomarker model.Conclusion: Brassicasterol might be a relevant additional biomarker in AD.