Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry
Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry
复制标题
DOI：
10.1111/j.1600-0722.2006.00261.x
复制
发表时间：
2006-02-01
影响因子：
1.9
通讯作者：
Ohshima, H
中科院分区：
文献类型：
--
作者：
Tate, Y;Yoshiba, K;Ohshima, H
牙髓反应镓铝砷化物（GaAlAs）激光照射的牙齿仍有待阐明。本研究旨在使用标记成熟和新分化的成牙本质细胞的热休克蛋白（HSP）-25的免疫组织化学来评估GaAlAs激光对成牙本质细胞的影响。用功率为0.5- 1.5W的激光照射8周龄Wistar大鼠右上第一磨牙近中面180 s。在照射后6 h至30 d间隔灌注固定动物。在6 h至7 d，HSP-25免疫反应的强度被发现在冠状面成牙本质细胞层中以能量依赖的方式被干扰。30 d时，牙髓中有大量的第三层牙本质形成，有或无骨样组织形成。统计学分析表明，新的硬组织所占的面积显着更广泛的1.5 W激光标本比0.5 W激光标本。一个强烈的HSP-25免疫反应被认为是在成牙本质细胞下面的三级牙本质，而免疫反应弱周围的骨样组织。提示GaAlAs激光可能通过影响成牙本质细胞的分泌活性而诱导三级牙本质的形成。然而，更高的能量可能会导致牙髓发生不可逆的变化，通常会导致髓内骨样组织的形成。
Pulpal responses to gallium-aluminum-arsenide (GaAlAs) laser irradiation applied to the tooth remains to be elucidated. This study aimed to evaluate the effect of the GaAlAs laser on odontoblasts using immunohistochemistry for heat-shock protein (HSP)-25, which labels mature and newly differentiated odontoblasts. The mesial surface of the upper right first molar of 8-wk-old Wistar rats was lased at an output power of 0.5-1.5 W for 180 s. The animals were perfusion-fixed at intervals of 6 h to 30 d after irradiation. At 6 h to 7 d, the intensity of HSP-25-immunoreactivity was found to be disturbed in the coronal odontoblast-layer in an energy-dependent manner. At 30 d, tertiary dentin with/without bone-like tissue was formed abundantly in the dental pulp. Statistical analysis revealed that the area occupied by the new hard tissues was significantly wider in 1.5 W-lased specimens than in 0.5 W-lased specimens. An intense HSP-25 immunoreactivity was seen in the odontoblasts underlying the tertiary dentin, whereas immunoreactivity was weak around the bone-like tissue. It was concluded that the GaAlAs laser may induce the formation of tertiary dentin by influencing the secretory activity of odontoblasts. However, higher energies may cause irreversible changes to the pulp, often leading to the formation of an intrapulpal bone-like tissue.