Genome-wide identification, expression and functional analysis of the phosphofructokinase gene family in Chinese white pear (Pyrus bretschneideri)
Genome-wide identification, expression and functional analysis of the phosphofructokinase gene family in Chinese white pear (Pyrus bretschneideri)
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中国白梨磷酸果糖激酶基因家族的全基因组鉴定、表达及功能分析
DOI：
10.1016/j.gene.2019.03.005
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发表时间：
2019-06-20
期刊：
影响因子：
3.5
通讯作者：
Wu, Jun
中科院分区：
文献类型：
--
作者：
Lu, Hongmei;Li, Jiaming;Wu, Jun
磷酸果糖激酶在植物糖代谢中起着重要作用。植物具有两种类型的磷酸果糖激酶蛋白，用于磷酸化果糖-6-磷酸，焦磷酸依赖性果糖-6-磷酸转移酶（PFP）和ATP依赖性磷酸果糖激酶（PFK）。到目前为止，梨中磷酸果糖激酶蛋白的基因进化、表达模式和功能尚不清楚。本研究在梨中鉴定了14个磷酸果糖激酶基因。系统进化树显示，磷酸果糖激酶基因家族可分为两个亚家族，其中10个基因属于PbPFK亚家族，4个基因属于PbPFP亚家族。梨等6种植物的磷酸果糖激酶基因具有保守的基序和外显子数目。进化分析表明，WGD/Segmental和分散重复是梨等6种植物磷酸果糖激酶基因扩增的主要模式。对所有磷酸果糖激酶基因的顺式调控元件序列进行分析，发现所有梨磷酸果糖激酶基因的启动子中均存在光调控和MYB结合位点，表明磷酸果糖激酶可能受光和MYB转录因子的调控。基因表达谱分析表明，PbPFP 1与山梨醇含量的表达模式相似，这表明PbPFP 1在果实发育过程中对糖的积累有重要贡献。进一步的功能分析表明，磷酸果糖激酶基因PbPFP 1定位于质膜区室，表明PbPFP 1在质膜中具有功能。瞬时转化的PbPFP 1在梨果实中导致显着增加的果糖和山梨醇相比，控制。本研究为揭示梨果实中磷酸果糖激酶的基因表达模式及其在糖积累中的重要潜在功能提供了重要信息，有助于丰富对糖相关生物学途径的认识，为果实品质改良奠定分子基础。
Phosphofructokinase plays an essential role in sugar metabolism in plants. Plants possess two types of phosphofructokinase proteins for phosphorylation of fructose-6-phosphate, the pyrophosphate-dependent fructose-6-phosphate phosphotransferase (PFP), and the ATP-dependent phosphofructokinase (PFK). Until now, the gene evolution, expression patterns, and functions of phosphofructokinase proteins were unknown in pear. In this report, 14 phosphofructokinase genes were identified in pear. The phylogenetic tree indicated that the phosphofructokinase gene family could be grouped into two subfamilies, with 10 genes belonging to the PbPFK subfamily, and 4 genes belonging to the PbPFP subfamily. Conserved motifs and exon numbers of the phosphofructokinase were found in pear and other six species. The evolution analysis indicated that WGD/Segmental and dispersed duplications were the main duplication models for the phosphofructokinase genes expansion in pear and other six species. Analysis of cis-regulatory element sequences of all phosphofructokinase genes identified light regulation and the MYB binding site in the promoter of all pear phosphofructokinase genes, suggesting that phosphofructokinase might could be regulated by light and MYB transcription factors (TFs). Gene expression patterns revealed that PbPFP1 showed similar pattern with sorbitol contents, suggesting important contributions to sugar accumulation during fruit development. Further functional analysis indicated that the phosphofructokinase gene PbPFP1 was localized on plasma membrane compartment, indicating that PbPFP1 had function in plasma membrane. Transient transformation of PbPFP1 in pear fruits led to significant increases of fructose and sorbitol compared to controls. Overall, our study provides important insights into the gene expression patterns and important potential functions of phosphofructokinase for sugar accumulation in pear fruits, which will help to enrich understanding of sugar-related bio-pathways and lay the molecular basis for fruit quality improvement.