Sequencing of hsp65 distinguishes among subsets of the Mycobacterium avium complex
Sequencing of hsp65 distinguishes among subsets of the Mycobacterium avium complex
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DOI：
10.1128/jcm.44.2.433-440.2006
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发表时间：
2006-02-01
影响因子：
9.4
通讯作者：
Behr, MA
中科院分区：
文献类型：
--
作者：
Turenne, CY;Semret, M;Behr, MA
鸟分枝杆菌复合体由流行病学上不同的亚群组成。这些亚群的分类是复杂的，由许多因素，包括模糊的结果与表型和遗传测定和最近的升值，人类和禽类菌株似乎是不同的。在先前的工作中，基于hsp 65基因的441-bp部分的测序已被证明能有效地对分枝杆菌属内的分离株进行分类，但对区分分枝杆菌属的成员提供低分辨率。avium complex.因此，在本研究中，我们针对hsp 65基因的3'区，以确定它是否可以有效地区分M。在种和亚种水平上对禽流感复合群分离株进行了研究。针对该靶标设计的引物一致地生成了所有归类为M的微生物的扩增子。avium complex.序列分析表明，M. intracellulare与M. avium微生物，并获得了不同的sequevars为M.鸟类亚群，包括M.鸟亚种avium（鸟型）、M.鸟亚种hominissuis和M.鸟亚种副结核病此外，序列差异有助于区分牛和羊的M。鸟亚种副结核病一个独特的配置文件为M.鸟亚种未获得森林霉素。这些结果表明，对hsp 65基因3'端序列进行测序可以简单而明确地区分M. avium complex.
The Mycobacterium avium complex consists of epidemiologically distinct subsets. The classification of these subsets is complicated by a number of factors, including the ambiguous results obtained with phenotypic and genetic assays and the recent appreciation that human and avian strains appear to be distinct. In previous work sequencing based on a 441-bp portion of the hsp65 gene has proven to efficiently classify isolates within the Mycobacterium genus but provides low resolution for distinguishing among members of the M. avium complex. Therefore, in this study, we have targeted the more variable 3' region of the hsp65 gene to determine whether it can effectively discriminate M. avium complex isolates at the levels of species and subspecies. Primers designed for this target consistently generated amplicons for all organisms classified as M. avium complex. Sequences obtained indicate that M. intracellulare is genetically divergent from M. avium organisms, and distinct sequevars were obtained for M. avium subsets, including M. avium subsp. avium (bird type), M. avium subsp. hominissuis, and M. avium subsp. paratuberculosis. In addition, sequence differences served to distinguish bovine from ovine strains of M. avium subsp. paratuberculosis. A unique profile for M. avium subsp. silvaticum was not obtained. These results indicate that sequencing the 3' region of the hsp65 gene can simply and unambiguously distinguish species and subspecies of the M. avium complex.