Changes in microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish
Changes in microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish
复制标题
DOI：
10.1016/j.aquatox.2004.02.002
复制
发表时间：
2004-05-28
影响因子：
4.5
通讯作者：
Takamura, N
中科院分区：
文献类型：
--
作者：
Jang, MH;Ha, K;Takamura, N
与直接暴露于食植鱼类（鲢），增加特定的质量微囊藻毒素生产发生在三个单克隆铜绿微囊藻菌株（NIES 44，88和99）。NIES-44藻株在H.白鲢的含量比对照组高50倍以上（对照组的平均值为16.2 μ g（-1）-干细胞，而处理组的平均值为878.6 μ g（-1）-干细胞）。在暴露于H.与对照组相比（对照组的平均值为553，而处理组的平均值为5145 μ g（-1）-干细胞）。各菌株的藻毒素水平在对照组和H.白鲢处理组（NIES 44和88组P < 0.01; NIES 99组P < 0.05）。微囊藻毒素对杂食性异育银鲫的反应弱于对杂食性异育银鲫的反应。尽管暴露于鱼类的所有菌株中的水平均高于对照组，但NIES 44的微囊藻毒素产量在对照组和杂食性鱼类处理组之间存在显著差异（对照组的平均值为6.9，治疗组为41.5 μ g（-1）-干细胞; P < 0.01）和NIES 88（平均值为359.8对480.4 μ g g（-1）干细胞; P < 0.05）。在试验的最后一天，在鱼的粪便和肠道内容物中都观察到微囊藻细胞，在两种鱼的身体组织（0.6至2.5 μ g（-1）-干重）和粪便中也检测到微囊藻毒素，尽管在三种微囊藻培养物中98%的鱼体重减轻（平均值+/- S.E.）。鱼类生长率与M.铜绿假单胞菌;每天-0.90 +/-0.06%，n = 96）。这项研究表明，几种M.铜绿假单胞菌菌株增加毒素生产时，暴露于鱼类，特别是食植物性物种，即使鱼类似乎不积极饲料有毒微囊藻，并支持这一假设，即这种反应是鱼类诱导的防御介导的物理接触与喂养或化学线索（如利它素）。(C)2004 Elsevier B. V.保留所有权利。
With direct exposure to phytoplanktivorous fish (Hypophthalmichthys molitrix), increased mass-specific microcystin production occurred in three monoclonal Microcystis aeruginosa strains (NIES 44, 88 and 99). Total mass-specific microcystin content of NIES 44 exposed to H. molitrix was over 50 times higher than controls (a mean value of 16.2 mug g(-1)-dry cell in controls versus 878.6 mug g(-1)-dry cell in treatments). Up to nine times higher microcystin levels were detected in NIES 88 exposed to H. molitrix compared to controls (a mean value of 553 in controls versus 5145 mug g(-1)-dry cell in treatments). The microcystin levels of all strains were significantly different between controls and H. molitrix treatments (P < 0.01 for NIES 44 and 88; P < 0.05 for NIES 99). The microcystin response to the omnivorous Carassius gibelio langsdorfi was weaker than that of H. molitrix, though the levels in all strains exposed to the fish were higher than in controls and a significant difference in microcystin production between controls and omnivorous fish treatments occurred for NIES 44 (a mean value of 6.9 in controls versus 41.5 mug g(-1)-dry cell in treatments; P < 0.01) and NIES 88 (a mean value of 359.8 versus 480.4 mu g g(-1)-dry cell; P < 0.05). Microcystis cells were observed in the both fish faeces and gut contents, and microcystin was also detected in the body tissues (from 0.6 to 2.5 mug g(-1)-dry weight) and faeces of both fish species on the final day of experiment, although 98% of fish in three strains of Microcystis cultures had lost weight (mean +/- S.E. fish growth rate with M. aeruginosa; -0.90 +/- 0.06% per day, n = 96). This study showed that several M. aeruginosa strains increased toxin production when exposed to fish, especially phytoplanktivorous species, even though fish appeared not to feed vigorously on toxic Microcystis, and supports the hypothesis that this response is a fish-induced defence mediated by physical contact associated with feeding or by chemical cues (e.g. kairomones). (C) 2004 Elsevier B.V. All rights reserved.