Afferent and efferent connections of the parvicellular subdivision of iNTS: defining a circuit involved in taste aversion learning.
Afferent and efferent connections of the parvicellular subdivision of iNTS: defining a circuit involved in taste aversion learning.
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iNTS 小细胞细分的传入和传出连接：定义涉及味觉厌恶学习的电路。
DOI：
10.1016/j.bbr.2004.01.027
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Bernstein,IleneL
中科院分区：
文献类型：
--
作者：
Spray,KristinaJ;Bernstein,IleneL
条件性味觉厌恶（CTA）表达与脑干中被鉴定为孤束中间核（iNTSpc）的小细胞亚群的区域中Fos样免疫反应性（FLI）的强烈增加相关。为了鉴定响应于CTA表达而显示强FLI的iNTSpc中细胞的投射和来自iNTSpc中细胞的投射，使用顺行和逆行束追踪。适当时，在接受CTA训练以及示踪剂注射并再次暴露于CS味道的动物中，将束示踪与FLI的双标记相结合。关于传入投射，iNTSpc从杏仁核接收强的、直接的、同侧投射，并且纤维末端的分布与CTA表达后表达FLI的细胞的分布产生惊人的匹配。至于传出投射，iNTSpc中的这些细胞的特征在于混合而不是均匀的投射模式。这些细胞的靶点包括脑桥和前脑以及局部髓质部位，已知所有这些部位都参与胃肠道功能。因此，这些细胞的激活可能提供了一个回路，通过该回路，胃肠道/内脏反应被协调为条件性厌恶的一个组成部分。
Conditioned taste aversion (CTA) expression is associated with strong increases in Fos-like immunoreactivity (FLI) in a region of the brainstem identified as the parvicellular subdivision of the intermediate nucleus of the solitary tract (iNTSpc). To identify the projections to and from cells in iNTSpc which display strong FLI in response to expression of a CTA, anterograde and retrograde tract tracing was used. When appropriate, tract tracing was combined with double labeling for FLI in animals which received CTA training as well as tracer injections and were re-exposed to the CS taste. With respect to afferent projections, iNTSpc receives a strong, direct, ipsilateral projection from amygdala and the distribution of the fiber terminals yields a striking match to that of cells expressing FLI after CTA expression. As for efferent projections, these cells in iNTSpc are characterized by a mixed, rather than homogeneous, projection pattern. Targets of these cells include pons and forebrain as well as local medullary sites, all of which are known to be involved in gastrointestinal function. Thus, activation of these cells may provide a circuit through which gastrointestinal/visceral responses are coordinated as a component of the conditioned aversion.