MRI of prostate stem cell antigen expression in prostate tumors
MRI of prostate stem cell antigen expression in prostate tumors
复制标题
前列腺肿瘤中前列腺干细胞抗原表达的 MRI
DOI：
10.2217/nnm.11.147
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发表时间：
2012-05-01
期刊：
影响因子：
5.5
通讯作者：
Larson, Andrew C.
中科院分区：
文献类型：
--
作者：
Ren, Jing;Zhang, Zhuoli;Larson, Andrew C.
背景：前列腺干细胞抗原（PSCA）在前列腺癌细胞表面广泛过表达。材料和方法：将抗人前列腺特异性抗原（PSCA）单克隆抗体（mAb 7 F5）与Fe 3 O 4/Au（GoldMag）纳米颗粒结合，作为PSCA特异性分子MRI探针（mAb 7F5@GoldMag）用于前列腺癌细胞的体内检测。首先，评估抗体固定化对结合的功效。接着，对PC-3（具有PSCA过表达的人前列腺癌细胞系）荷瘤小鼠注射mAb 7F5@GoldMag用于MRI测量，同时使用与颗粒结合的小鼠抗人IgG（IgG@GoldMag）作为非特异性对照。在注射这些探针之前和之后6、12和24 h进行MRI检查;测量肿瘤内的T2加权信号强度。结果如下：用光学图像和MRI验证mAb 7F5@GoldMag探针与PC-3肿瘤的靶向结合;未观察到非特异性IgG@GoldMag探针的选择性结合。结论：MRI测量表明这种新方法用于前列腺肿瘤的靶向分子成像具有良好的效果。
Background: The prostate stem cell antigen (PSCA) is broadly overexpressed on the surface of prostate cancer cells. Materials & methods: Anti-human PSCA monoclonal antibody (mAb 7F5) was bound to Fe3O4/Au (GoldMag) nanoparticles to serve as a PSCA-specific molecular MRI probe (mAb 7F5@GoldMag) for in vivo detection of prostate cancer cells. First, the efficacy of the antibody immobilization for the binding was assessed. Next, PC-3 (human prostate cancer cell line with PSCA overexpression) tumor-bearing mice were injected with mAb 7F5@GoldMag for MRI measurements while using mouse anti-human IgG bound to the particles (IgG@GoldMag) to serve as a nonspecific control. MRI examinations were conducted before and after injection of these probes at 6, 12 and 24 h; T2-weighted signal intensity within the tumors was measured. Results: Targeted binding of the mAb 7F5@GoldMag probe to PC-3 tumors was verified with optical images and MRI; selective binding was not observed for the nonspecific IgG@GoldMag probe. Conclusion: MRI measurements suggest the promising efficacy of this new approach for targeted molecular imaging of prostate tumors.