J4 at sweet 16: A new wrinkle?
J4 at sweet 16: A new wrinkle?
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DOI：
10.1162/neco.2007.19.11.2865
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发表时间：
2007-11-01
影响因子：
2.9
通讯作者：
Mel, Bartlett W.
中科院分区：
文献类型：
--
作者：
Behabadi, Bardia F.;Mel, Bartlett W.
隔室模型为了解单个神经元的信息处理功能提供了一个主要的来源。在过去的15年里，最广泛使用的神经元形态之一是被称为“J4”的细胞，这是一种来自猫视皮质的第5层锥体细胞，最初在Douglas，Martin和Whitteridge(1991)中描述。自那以后，该细胞已经出现在至少28篇已发表的隔室建模研究中，包括本杂志上的几篇。在最近检查为什么我们不能重现某些涉及起源于远端基底树突的信号衰减的体外数据时，我们发现J4的直径测量的显著波动导致了瓶颈效应，从而增加了远端输入电阻，并显著减少了远端位置和细胞体之间的电压转移。在平滑这些直径波动后，使J4与其他第5层锥体神经元的重建更加一致，我们发现，在一些分支的某些位置，传输到细胞体(V-远端/V-胞体)的稳态电压信号的衰减减少了60%(对应于相同远端去极化时在胞体的电压响应增加了2.5倍)，平均减少了30%(对应于躯体反应增加了45%)。这种大小的变化可能会导致某些类型的隔室建模研究产生不同的结果。J4形态的平滑版本可在http://lnc.usc.edu/j4-smooth/.上在线获得
Compartmental models provide a major source of insight into the information processing functions of single neurons. Over the past 15 years, one of the most widely used neuronal morphologies has been the cell called "j4," a layer 5 pyramidal cell from cat visual cortex originally described in Douglas, Martin, and Whitteridge (1991). The cell has since appeared in at least 28 published compartmental modeling studies, including several in this journal. In recently examining why we could not reproduce certain in vitro data involving the attenuation of signals originating in distal basal dendrites, we discovered that pronounced fluctuations in the diameter measurements of j4 lead to a bottlenecking effect that increases distal input resistances and significantly reduces voltage transfer between distal sites and the cell body. Upon smoothing these diameter fluctuations, bringing j4 more in line with other reconstructions of layer 5 pyramidal neurons, we found that the attenuation of steady-state voltage signals traveling to the cell body (V-distal/V-soma) was reduced by 60% at some locations in some branches (corresponding to a 2.5-fold increase in the voltage response at the soma for the same distal depolarization) and by 30% on average (corresponding to a 45% increase in somatic response). Changes of this magnitude could lead to different outcomes in some types of compartmental modeling studies. A smoothed version of the j4 morphology is available online at http://lnc.usc.edu/j4-smooth/.