K+ Release Assay and K+ Measurement in Oocyte Assay.
K+ Release Assay and K+ Measurement in Oocyte Assay.
复制标题
卵母细胞测定中的 K 释放测定和 K 测量。
DOI：
10.21769/bioprotoc.3802
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发表时间：
2020
期刊：
影响因子：
0.8
通讯作者：
Hong Li
中科院分区：
文献类型：
--
作者：
Hong Li
非洲爪哇卵母细胞是植物膜转运蛋白外源表达和功能鉴定的有力系统。到目前为止，通过双电极电压钳技术已经在卵母细胞表达系统中发现了许多钾转运体和钾通道。K+/H+反转运蛋白，尤其是电子中和型转运蛋白的特性很难确定。K+外流检测系统无需双电极电压钳技术即可轻松、快速、大规模地测量转运蛋白活性。本方案描述了一种测量卵母细胞表达系统中钾转运体外流活性的技术。
The Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a number of potassium transporters and channels have been identified in oocytes expression system by the two-electrode voltage clamp technology. It is difficult to characterize K+/H+ anti-transporters, especially, electroneutral transporter. The K+ efflux assay system enables easy, fast, large-scale measurement of the transporters activity without two-electrode voltage clamp technology. This protocol describes a technique to measure the efflux activity of potassium transporter in oocytes expressing system.
影响因子：
11.6
作者：
CAO, YW;ANDEROVA, M;SCHROEDER, JI
通讯作者：
SCHROEDER, JI