Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.
Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.
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DOI：
10.12659/msm.881759
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Lin P
中科院分区：
文献类型：
--
作者：
Lan F;Liu K;Zhang J;Qi Y;Li K;Lin P
人们普遍认为，辅助 T 2 (Th2) 细胞、Th17 细胞及其细胞因子协调了哮喘的特征。然而，大多数关于哮喘机制的研究都使用单一过敏原激发模型。事实上，人类同时暴露于多种过敏原，而复杂过敏原暴露引发哮喘的机制尚不清楚。为了探讨如果哮喘患者重新暴露于另一种过敏原，该机制是否会改变，我们将鸡蛋清蛋白（OVA）诱导的哮喘小鼠暴露于屋尘螨（HDM）。 HE染色分析各亚组小鼠肺组织病理变化：对照组、单独HDM组、单独OVA组和OVA+HDM组。定量PCR检测Th1、Th2、Th17相关基因mRNA表达量；通过 ELISA 或免疫组织化学测定相关细胞因子。与单独OVA组相比，OVA+HDM组炎症细胞浸润更严重，Th17和Th2相关基因mRNA表达增加，Th17相关细胞因子表达增加。而OVA+HDM组Th2相关细胞因子升高，与单独OVA组相比无显着差异。我们发现 Th17 反应与暴露于 HDM 的 OVA 诱导的哮喘小鼠的炎症有关。当 OVA 诱导的哮喘小鼠再次暴露于 HDM 时，其病理机制与单独暴露于 OVA 不同。 HDM，室内过敏原，可能是哮喘治疗的重要干扰因素。这将为未来哮喘治疗的发展提供重要方向。
It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM). HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry. The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group. We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.
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DOI：
10.1084/jem.20082771
发表时间：
2009-09-28
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Klotz L;Burgdorf S;Dani I;Saijo K;Flossdorf J;Hucke S;Alferink J;Nowak N;Beyer M;Mayer G;Langhans B;Klockgether T;Waisman A;Eberl G;Schultze J;Famulok M;Kolanus W;Glass C;Kurts C;Knolle PA
通讯作者：
Knolle PA
影响因子：
5.6
作者：
Medeiros, K. C. P.;Faustino, L.;Russo, M.
通讯作者：
Russo, M.
DOI：
10.1152/ajplung.00142.2009
发表时间：
2009-10-01
影响因子：
4.9
作者：
Labonte, Isabelle;Hassan, Muhannad;Martin, James G.
通讯作者：
Martin, James G.
影响因子：
--
作者：
Chen, Zhi;Tato, Cristina M.;O'Shea, John J.
通讯作者：
O'Shea, John J.
影响因子：
4.4
作者：
Cheung, Phyllis F. Y.;Wong, Chun K.;Lam, Christopher W. K.
通讯作者：
Lam, Christopher W. K.