Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis
Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis
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DOI：
10.1053/gast.1996.v110.pm8964405
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发表时间：
1996-06-01
期刊：
影响因子：
29.4
通讯作者：
Toh, BH
中科院分区：
文献类型：
--
作者：
Martinelli, TM;vanDriel, IR;Toh, BH
背景与目的：胸腺切除术第3天引起的小鼠自身免疫性胃炎的特点是细胞浸润和循环胃氢/钾腺苷三磷酸酶自身抗体。方法：取胸腺切除术后第3天BALB/c小鼠的胃和血液样本进行分析。结果：在第4周，胃浸润物由巨噬细胞和CD4(+) T细胞组成，并伴有胃上皮细胞上主要组织相容性复合体II类的表达。粘膜B细胞在4周时较少，在8周时丰富，与胃氢/钾腺苷三磷酸酶自身抗体的峰值一致，CD8(+) T细胞在12周期间轻微增加，病变胃的单核细胞将胃炎转移到nu/nu受体，在4周时，白细胞介素2,3,5,6和10；干扰素γ;肿瘤坏死因子；结论：自身免疫性胃炎早期病变由巨噬细胞和CD4(+) T细胞组成，胃上皮细胞主要表达组织相容性复合体II类。我们的结果与CD4(+) T细胞产生Th1-和th2型细胞因子混合介导的病变一致。
Background & Aims: Murine autoimmune gastritis, induced by day-3 thymectomy, is characterized by cellular infiltrates and circulating autoantibodies to gastric hydrogen/potassium adenosine triphosphatase. The aim of this study was to analyze the cellular infiltrates and cytokines in autoimmune gastritis, Methods: Stomachs and blood samples from day-3 thymectomized BALB/c mice were obtained from 2 to 12 weeks after thymectomy for analysis, Results: At 4 weeks, the gastritic infiltrates were composed of macrophages and CD4(+) T cells, accompanied by major histocompatibility complex class II expression on gastric epithelial cells. Mucosal B cells, scant at 4 weeks, were abundant at 8 weeks, coincident with the peaking of autoantibodies to gastric hydrogen/potassium adenosine triphosphatase, CD8(+) T cells increased marginally during the 12 weeks, Mononuclear cells from diseased stomachs transferred gastritis to nu/nu recipients, At 4 weeks, interleukins 2, 3, 5, 6, and 10; interferon gamma; tumor necrosis factor alpha; and granulocyte-macrophage colony-stimulating factor were detected in gastritic mucosa, but interleukin 4 was not, Conclusions: The early lesion of autoimmune gastritis is composed of macrophages and CD4(+) T cells with major histocompatibility complex class II expression in gastric epithelial cells. Autoantibody production is a late event, Our results ave consistent with a lesion mediated by CD4(+) T cells producing a mix of Th1- and Th2-type cytokines.