Non-Coding RNAs in the Transcriptional Network That Differentiates Skeletal Muscles of Sedentary from Long-Term Endurance- and Resistance-Trained Elderly.
Non-Coding RNAs in the Transcriptional Network That Differentiates Skeletal Muscles of Sedentary from Long-Term Endurance- and Resistance-Trained Elderly.
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DOI：
10.3390/ijms22041539
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发表时间：
2021-02-03
影响因子：
5.6
通讯作者：
Zucchini C
中科院分区：
文献类型：
--
作者：
De Sanctis P;Filardo G;Abruzzo PM;Astolfi A;Bolotta A;Indio V;Di Martino A;Hofer C;Kern H;Löfler S;Marcacci M;Marini M;Zampieri S;Zucchini C
在之前的一项研究中，研究人员比较了久坐不动的老年人和具有高强度、终身运动训练记录的同龄运动员的股外侧肌的整个转录组——这两组代表了身体活动量表上的两个极端。运动训练使骨骼肌能够通过诱导广泛的适应来对抗与年龄相关的肌肉减少症，这是由涉及能量处理、蛋白质平衡、细胞骨架组织、炎症控制和细胞衰老的蛋白质编码基因的表达维持的。在先前研究的基础上，我们研究了参与基因表达协调的非编码rna网络，并鉴定了差异表达的微非编码rna和长非编码rna及其一些可能的靶点和作用。所有非编码rna的无监督分层聚类分析能够区分久坐和训练的个体，无论运动类型如何。通过KEGG分析对已验证的差异表达miRNA靶点进行分组，这些靶点指向涉及细胞周期、细胞骨架控制、寿命和许多信号通路的功能区域，包括amp活化蛋白激酶（AMPK）和哺乳动物雷帕霉素靶点（mTOR），这些靶点已被证明在高强度终身运动训练的调节作用中起关键作用。对差异表达的长链非编码rna的分析发现了转录网络，包括lncrna、mirna和mrna，这些转录网络影响的过程与运动训练的有益作用一致。
In a previous study, the whole transcriptome of the vastus lateralis muscle from sedentary elderly and from age-matched athletes with an exceptional record of high-intensity, life-long exercise training was compared—the two groups representing the two extremes on a physical activity scale. Exercise training enabled the skeletal muscle to counteract age-related sarcopenia by inducing a wide range of adaptations, sustained by the expression of protein-coding genes involved in energy handling, proteostasis, cytoskeletal organization, inflammation control, and cellular senescence. Building on the previous study, we examined here the network of non-coding RNAs participating in the orchestration of gene expression and identified differentially expressed micro- and long-non-coding RNAs and some of their possible targets and roles. Unsupervised hierarchical clustering analyses of all non-coding RNAs were able to discriminate between sedentary and trained individuals, regardless of the exercise typology. Validated targets of differentially expressed miRNA were grouped by KEGG analysis, which pointed to functional areas involved in cell cycle, cytoskeletal control, longevity, and many signaling pathways, including AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR), which had been shown to be pivotal in the modulation of the effects of high-intensity, life-long exercise training. The analysis of differentially expressed long-non-coding RNAs identified transcriptional networks, involving lncRNAs, miRNAs and mRNAs, affecting processes in line with the beneficial role of exercise training.
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DOI：
10.1038/nrg3355
发表时间：
2013-02
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
64.8
作者：
BURNS, K;DUGGAN, B;MICHALAK, M
通讯作者：
MICHALAK, M
影响因子：
48
作者：
Ebert, Margaret S.;Neilson, Joel R.;Sharp, Phillip A.
通讯作者：
Sharp, Phillip A.
影响因子：
4.6
作者：
Flowers, Elena;Won, Gloria Y.;Fukuoka, Yoshimi
通讯作者：
Fukuoka, Yoshimi
影响因子：
3.9
作者：
Chang, Yun-Ching;Liu, Hung-Wen;Chang, Sue-Joan
通讯作者：
Chang, Sue-Joan