CYTOPLASMIC INHERITANCE OF CHLORAMPHENICOL RESISTANCE IN MOUSE TISSUE-CULTURE CELLS
CYTOPLASMIC INHERITANCE OF CHLORAMPHENICOL RESISTANCE IN MOUSE TISSUE-CULTURE CELLS
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DOI：
10.1073/pnas.71.5.1681
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
EISENSTA.JM
中科院分区：
文献类型：
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作者：
BUNN, CL;WALLACE, DC;EISENSTA.JM
从小鼠A9细胞中分离出一株氯霉素耐药突变体，将其去核并与有核的氯霉素敏感小鼠细胞系融合。所产生的融合产物细胞质杂交（或“细胞质杂交”）被选择为对氯霉素具有抗性的，并且具有氯霉素敏感亲本的核标记和染色体补体。这些杂交出现在每104个细胞电镀2-8个的高频。在相同的选择条件下，两个亲本都没有产生任何菌落。去核的氯霉素敏感细胞片段与氯霉素敏感细胞融合后未产生耐药菌落，表明氯霉素耐药不是由于细胞质与细胞核比例的增加。此外，抗性和敏感有核细胞之间的融合产生抗性杂交种的频率比抗性杂交种低100倍。因此，这些稳定的耐氯霉素杂交体是由耐氯霉素细胞质与氯霉素敏感细胞融合而成的。因此，我们认为氯霉素耐药性是该小鼠细胞系的细胞质遗传特征。
A chloramphenicol-resistant mutant, isolated from mouse A9 cells, was enucleated and fused with a nucleated chloramphenicol-sensitive mouse cell line. Resultant fusion products, cytoplasmic hybrids (or “cybrids”), were selected as resistant to chloramphenicol, and had the nuclear markers and chromosome complement of the chloramphenicol-sensitive parent. These cybrids appeared at the high frequency of 2-8 per 104cells plated. Neither parent produced any colonies when plated under identical selective conditions. Fusion between enucleated chloramphenicol-sensitive cell fragments and the chloramphenicol-sensitive cell produced no resistant colonies, suggesting that chloramphenicol resistance is not due to an increase in the ratio of cytoplasm to nucleus. Furthermore, fusions between resistant and sensitive nucleated cells produced resistant hybrids at a frequency 100 times less than that of resistant cybrids. Thus, these stable chloramphenicol-resistant cybrids result from the fusion of a chloramphenicol-resistant cytoplasm with a chloramphenicol-sensitive cell. It is proposed, therefore, that chloramphenicol resistance is a cytoplasmically inherited characteristic in this mouse cell line.