Vascular Smooth Muscle Cell-Derived Exosomal MicroRNAs Regulate Endothelial Cell Migration Under PDGF Stimulation
Vascular Smooth Muscle Cell-Derived Exosomal MicroRNAs Regulate Endothelial Cell Migration Under PDGF Stimulation
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DOI：
10.3390/cells9030639
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发表时间：
2020-03-01
期刊：
影响因子：
6
通讯作者：
Kang, Hara
中科院分区：
文献类型：
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作者：
Heo, Jeongyeon;Yang, Hee Cheol;Kang, Hara
血管平滑肌细胞（VSMCs）和内皮细胞（ECs）之间的细胞间通讯对于维持血管内环境的稳定是必不可少的。外泌体的存在，最近发现的球员在血管细胞通讯，已与血管疾病的进展。然而，在血管发病过程中，外泌体介导的信号如何影响血管细胞功能的详细机制还有待进一步了解。在这项研究中，我们研究了VSMC分泌的外泌体microRNA（miRNA）的表达及其与EC发病机制的功能相关性，包括它们在血小板衍生生长因子（PDGF）刺激等过程中的作用。我们观察到，PDGF刺激有助于改变外泌体miRNA从VSMC的释放;具体地，miR-1246、miR-182和miR-486在源自PDGF刺激的VSMC的外泌体中是缺乏的。这些外来体中miRNA表达的减少与EC迁移的增加相关。这些发现增加了我们对病理条件下外泌体介导的血管细胞之间串扰的理解。
Intercellular communication between vascular smooth muscle cells (VSMCs) and endothelial cells (ECs) is essential for the maintenance of vascular homeostasis. The presence of exosomes, a recently discovered player in vascular cell communication, has been associated with vascular disease progression. However, the detailed mechanism of how the signal mediated by exosomes affects the function of vascular cells during vascular pathogenesis is yet to be further understood. In this study, we investigated the expression of exosomal microRNAs (miRNAs) secreted by VSMCs and their functional relevance to ECs in pathogenesis, including their role in processes such as platelet-derived growth factor (PDGF) stimulation. We observed that PDGF stimulation contributes to a change in exosomal miRNA release from VSMCs; specifically, miR-1246, miR-182, and miR-486 were deficient in exosomes derived from PDGF-stimulated VSMCs. The reduced miRNA expression in these exosomes is associated with an increase in EC migration. These findings increase our understanding of exosome-mediated crosstalk between vascular cells under a pathological condition.