A vasculature-associated niche for undifferentiated spermatogonia in the mouse testis
A vasculature-associated niche for undifferentiated spermatogonia in the mouse testis
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DOI：
10.1126/science.1144885
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发表时间：
2007-09-21
期刊：
影响因子：
56.9
通讯作者：
Nabeshima, Yo-ichi
中科院分区：
文献类型：
--
作者：
Yoshida, Shosei;Sukeno, Mamiko;Nabeshima, Yo-ichi
哺乳动物精子发生基于高度有效的干细胞系统长时间产生大量精子，该系统依赖于特殊的微环境或生态位，但尚未确定。在这项研究中，使用延时成像的绿色荧光蛋白标记的未分化精原细胞（A（undiff））和三维重建，我们发现了一个偏见的定位Aundiff的血管网络和陪同Leydig和其他间质细胞，在完整的睾丸。分化的精原细胞离开这些生态位区域，分散在整个生精上皮的基底室。此外，A（undiff）重排伴随着血管的改变。我们提出，哺乳动物生殖系生态位的建立是由于脉管系统模式的形成。这与在果蝇或秀丽隐杆线虫中观察到的不同，它们在极化性腺内显示发育特定的生态位结构。
Mammalian spermatogenesis produces numerous sperm for a long period based on a highly potent stem cell system, which relies on a special microenvironment, or niche, that has not yet been identified. In this study, using time-lapse imaging of green fluorescent protein-labeled undifferentiated spermatogonia (A(undiff)) and three-dimensional reconstitution, we revealed a biased localization of Aundiff to the vascular network and accompanying Leydig and other interstitial cells, in intact testes. Differentiating spermatogonia left these niche regions and dispersed throughout the basal compartment of the seminiferous epithelium. Moreover, rearrangement of A(undiff) accompanied the vasculature alteration. We propose that the mammalian germline niche is established as a consequence of vasculature pattern formation. This is different from what is observed in Drosophila or Caenorhabditis elegans, which display developmentally specified niche structures within polarized gonads.