iTRAQ-based identification of differentially expressed proteins in ED rat model induced by cold stress combined with environmental oestrogen
iTRAQ-based identification of differentially expressed proteins in ED rat model induced by cold stress combined with environmental oestrogen
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基于iTRAQ鉴定冷应激联合环境雌激素诱导的ED大鼠模型差异表达蛋白
DOI：
10.1111/and.14382
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发表时间：
2022-01-29
期刊：
影响因子：
2.4
通讯作者：
Ruze, Abudureyimujiang
中科院分区：
文献类型：
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作者：
Ma, Wenjing;Zhang, Panpan;Ruze, Abudureyimujiang
我们的目的是通过 iTRAQ 结合 LC-MS/MS 来鉴定冷应激联合环境雌激素诱导的勃起功能障碍 (ED) 的血清生物标志物和关键蛋白。采用类雌激素饲料和冷刺激建立ED大鼠模型。利用iTRAQ结合LC-MS/MS和IPA生物分析平台对血清中差异表达蛋白进行筛选和分析。我们发现 35 个蛋白质在 ED 组中差异表达，其中 31 个上调蛋白质和 4 个下调蛋白质。差异蛋白的分子功能主要涉及肽链内切酶抑制剂和调节剂的激活。主要涉及的通路为FXR/RXR激活信号通路和急性期反应信号通路。此外，还获得了与 ED 相关的 3 个蛋白质标志物，即 C 反应蛋白（CRP）、T 激肽原 1 和载脂蛋白 H（APOH）。 ELISA结果显示，与对照组相比，ED组血清中HPX、TTR、APOH、RBP4、CRP、ORM1蛋白显着上调，而ANGPT1蛋白显着下调（p < 0.05）。最终获得冷应激联合环境激素诱导的ED候选血清标志物。我们的研究结果表明炎症和血管内皮功能的改变可能在ED的发生和发展中起关键作用。
We aim to identify serum biomarkers and key proteins of erectile dysfunction (ED) induced by cold stress combined with environmental oestrogen through iTRAQ combined with LC-MS/MS. ED rat model was established by using oestrogen-like feed and cold stimulation. Differentially expressed proteins in serum were screened and analysed with iTRAQ combined with LC-MS/MS and IPA bioanalysis platform. We found that 35 proteins were differentially expressed in the ED group, including 31 upregulated and four downregulated proteins. The molecular functions of differential proteins mainly involved the activation of endopeptidase inhibitor and regulator. The main pathways involved were the FXR/RXR activation signalling pathway and the acute phase response signalling pathway. Furthermore, three protein markers related to ED were obtained, namely C-reactive protein (CRP), T-kininogen 1 and apolipoprotein Protein H (APOH). ELISA results showed that compared with the control group, proteins of HPX, TTR, APOH, RBP4, CRP and ORM1 were significantly upregulated, but ANGPT1 protein was significantly downregulated in the serum of the ED group (p < 0.05). Conclusively, the candidate serum markers for ED induced by cold stress combined with environmental hormones were obtained. Our results indicate that inflammation and vascular endothelial function changes may play a key role in the occurrence and development of ED.