Effectiveness of Ultraviolet-C Light and a High-Level Disinfection Cabinet for Decontamination of N95 Respirators.
Effectiveness of Ultraviolet-C Light and a High-Level Disinfection Cabinet for Decontamination of N95 Respirators.
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DOI：
10.20411/pai.v5i1.372
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发表时间：
2020-01-01
影响因子：
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通讯作者：
Donskey, Curtis J
中科院分区：
文献类型：
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作者：
Cadnum, Jennifer L;Li, Daniel F;Donskey, Curtis J
背景：包括N95呼吸器在内的个人防护装备短缺是全球新冠肺炎大流行背景下的一个紧迫问题。个人防护用品的去污有助于维持充足的供应，但去污技术的效果尚不确定。方法：采用美国检测与材料学会标准定量载体纸片试验方法(ASTM E-2197-11)的修改，检测3种方法的有效性，包括紫外线-C(UV-C)光、产生雾化过氧乙酸和过氧化氢的高级消毒柜，以及70℃干热30分钟。我们评估了接种耐甲氧西林金黄色葡萄球菌(MRSA)和噬菌体MS2和Phi6的3种商用N95呼吸器的去污效果，后者是一种被包裹的RNA病毒，用作冠状病毒的替代品。结果：在UV-C盒中使用1分钟一次的UV-C或使用室内去污装置进行30分钟一次的UV-C治疗减少了污染，但不符合N95上所有站点病毒的消毒标准。该高级消毒柜对N95进行了有效的去污，并实现了延长31分钟的消毒周期。70℃干热30min对噬菌体的去污效果不佳。结论：UV-C可有效减少N95口罩的污染。然而，所研究的UV-C技术在所使用的测试条件下不符合预先建立的去污标准。高级消毒柜的消毒效果更好，符合消毒标准，周期更长。
BACKGROUND: Shortages of personal protective equipment (PPE) including N95 respirators are an urgent concern in the setting of the global COVID-19 pandemic. Decontamination of PPE could be useful to maintain adequate supplies, but there is uncertainty regarding the efficacy of decontamination technologies.METHODS: A modification of the American Society for Testing and Materials standard quantitative carrier disk test method (ASTM E-2197-11) was used to examine the effectiveness of 3 methods, including ultraviolet-C (UV-C) light, a high-level disinfection cabinet that generates aerosolized peracetic acid and hydrogen peroxide, and dry heat at 70°C for 30 minutes. We assessed the decontamination of 3 commercial N95 respirators inoculated with methicillin-resistant Staphylococcus aureus (MRSA) and bacteriophages MS2 and Phi6; the latter is an enveloped RNA virus used as a surrogate for coronaviruses. Three and 6 log10 reductions on respirators were considered effective for decontamination and disinfection, respectively.RESULTS: UV-C administered as a 1-minute cycle in a UV-C box or a 30-minute cycle by a room decontamination device reduced contamination but did not meet criteria for decontamination of the viruses from all sites on the N95s. The high-level disinfection cabinet was effective for decontamination of the N95s and achieved disinfection with an extended 31-minute cycle. Dry heat at 70°C for 30 minutes was not effective for decontamination of the bacteriophages.CONCLUSIONS: UV-C could be useful to reduce contamination on N95 respirators. However, the UV-C technologies studied did not meet pre-established criteria for decontamination under the test conditions used. The high-level disinfection cabinet was more effective and met criteria for disinfection with an extended cycle.