P53 protein accumulates in Cushings adenomas and invasive non-functional adenomas.
P53 protein accumulates in Cushings adenomas and invasive non-functional adenomas.
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P53 蛋白在库欣腺瘤和侵袭性非功能性腺瘤中积累。
DOI：
10.1210/jc.80.2.4
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发表时间：
1995
影响因子：
5.8
通讯作者：
R. Clayton
中科院分区：
文献类型：
--
作者：
N. Buckley;A. Bates;J. Broome;R. Strange;C. Perrett;C. Burke;R. Clayton
p53蛋白是细胞生长的负调节因子，在基因突变和/或缺失后的许多人类肿瘤的发病机制中起重要作用。我们筛选了大量的散发性垂体瘤p53蛋白的积累，提示基因突变。样本分为良性腺瘤（n = 95）和局部或远处浸润的浸润性肿瘤（n = 26）。所有主要的肿瘤类别都有代表性。使用多克隆CM-1和单克隆DO-7和PAb 1801抗体通过石蜡包埋切片的免疫组织化学检测推定的p53突变。将结果与正常死后垂体组织对照（n = 17）进行比较。p53蛋白在浸润性肿瘤中检出（16%），但仅在促肾上腺皮质激素瘤（2/4）和无功能性肿瘤（4/15）中检出。在非侵袭性腺瘤中，仅在ACTH分泌肿瘤中观察到蛋白质积聚，其中50%为阳性（16/32）。在任何对照组织中均未发现蛋白质蓄积。这些结果表明，p53蛋白的积累可能在库欣腺瘤的发展中发挥作用，并在非功能性肿瘤的侵袭性状态的进展。
The p53 protein, a negative regulator of cell growth, plays an important role in the pathogenesis of many human tumours following gene mutation and/or deletion. We screened a large number of sporadic pituitary tumours for p53 protein accumulation suggestive of gene mutation. Samples were divided into benign adenomas (n = 95) and invasive tumours with local or distant invasion (n = 26). All main tumour classes were represented. Putative p53 mutations were detected by immunohistochemistry on paraffin-embedded sections using polyclonal CM-1 and monoclonal DO-7 and PAb1801 antibodies. Results were compared to normal post-mortem pituitary tissue controls (n = 17). p53 protein accumulation was detected in invasive tumours (16%), but only in corticotrophinomas (2/4) and non-functional tumours (4/15). In non-invasive adenomas, protein accumulation was observed only in ACTH-secreting tumours where 50% were positive (16/32). No protein accumulation was identified in any control tissue. These results indicate that p53 protein accumulation may play a role in the development of Cushings adenomas and in the progression of non-functional tumours to the invasive state.