MYCN protein stability is a better prognostic indicator in neuroblastoma.
MYCN protein stability is a better prognostic indicator in neuroblastoma.
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MYCN 蛋白稳定性是神经母细胞瘤更好的预后指标
DOI：
10.1186/s12887-022-03449-1
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发表时间：
2022-07-11
期刊：
影响因子：
2.4
通讯作者：
中科院分区：
文献类型：
--
作者：
MYCN癌基因扩增与神经母细胞瘤治疗失败和预后不良相关。迄今为止，对于抗体较差的MYCN，大多数检测方法都侧重于DNA拷贝数，而不是真正发挥生物学功能的蛋白质表达。本研究旨在探索一种快速可靠的检测MYCN蛋白表达的方法，并评估其在预测预后方面的作用。免疫组化（IHC）检测MYCN蛋白表达，选择一种抗体进行进一步研究。我们将53例患者MYCN的免疫组化结果与MYCN荧光原位杂交（FISH）相关联，并鉴定了免疫组化的敏感性和特异性。在此基础上检测患者预后与MYCN蛋白表达的关系。FISH检测的MYCN扩增状态对INSS 3期患者最有价值。在53个样本的队列中，免疫组化检测结果与FISH结果的一致性为80.0-85.7%。进一步分析结果不一致的病例，我们发现MYCN扩增但蛋白表达低的肿瘤患者预后良好。相反，如果MYCN非扩增肿瘤患者MYCN蛋白阳性，则预后较差。MYCN蛋白水平比MYCN扩增状态更能预测神经母细胞瘤患者的预后。FISH和IHC联合验证了MYCN蛋白的稳定性，预测效果优于单一方法。在线版本包含补充材料，可在10.1186/s12887-022-03449-1获得。MYCN基因检测对INSS 3期患者预测预后最有价值。与基因状态相比，MYCN蛋白表达与预后更相关。结合FISH和IHC，可以鉴定MYCN蛋白的稳定性。在线版本包含补充材料，可在10.1186/s12887-022-03449-1获得。
MYCN oncogene amplification is associated with treatment failure and poor prognosis in neuroblastoma. To date, most detection methods of MYCN focus on DNA copy numbers instead of protein expression, which is the real one performing biological function, for poor antibodies. The current investigation was to explore a fast and reliable way to detect MYCN protein expression and evaluate its performance in predicting prognosis. Several MYCN antibodies were used to detect MYCN protein expression by immunohistochemistry (IHC), and one was chosen for further study. We correlated the IHC results of MYCN from 53 patients with MYCN fluorescence in situ hybridization (FISH) and identified the sensitivity and specificity of IHC. The relationship between patient prognosis and MYCN protein expression was detected from this foundation. MYCN amplification status detected by FISH was most valuable for INSS stage 3 patients. In the cohort of 53 samples, IHC test demonstrated 80.0–85.7% concordance with FISH results. Further analyzing those cases with inconsistent results, we found that patients with MYCN amplification but low protein expression tumors always had a favorable prognosis. In contrast, if patients with MYCN non-amplified tumors were positive for MYCN protein, they had a poor prognosis. MYCN protein level is better than MYCN amplification status in predicting the prognosis of neuroblastoma patients. Joint of FISH and IHC could confirm MYCN protein stability and achieve better prediction effect than the singular method. The online version contains supplementary material available at 10.1186/s12887-022-03449-1. The MYCN gene test is most valuable for INSS stage 3 patients to predict prognosis. Compared to gene status, MYCN protein expression is more relevant to prognosis. Combining FISH with IHC, MYCN protein stability could be identified. The online version contains supplementary material available at 10.1186/s12887-022-03449-1.
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