Dual roles for CND1 in maintenance of nuclear and chloroplast genome stability in plants.
Dual roles for CND1 in maintenance of nuclear and chloroplast genome stability in plants.
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DOI：
10.1016/j.celrep.2023.112268
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发表时间：
2023-03
期刊：
影响因子：
8.8
通讯作者：
Hong-Lei Jin;Sujuan Duan;Pengxiang Zhang;Ziyue Yang;Yunping Zeng;Ziqi Chen;Liu Hong;Mengshu Li-M
中科院分区：
文献类型：
--
作者：
Hong-Lei Jin;Sujuan Duan;Pengxiang Zhang;Ziyue Yang;Yunping Zeng;Ziqi Chen;Liu Hong;Mengshu Li-M
叶绿体和核基因组状态的协调对植物细胞功能至关重要。在这里，我们报告说，拟南芥叶绿体和核双定位蛋白1（CND1）保持基因组的稳定性，在叶绿体和核。CND 1定位于两个隔室，并且CND 1的完全丧失导致胚胎致死。CND1的部分缺失会干扰核细胞周期进程和光合活性。CND1与核复制前复合物和DNA复制起点结合并调节核基因组稳定性。在叶绿体中，CND1与叶绿体基因组稳定性调节因子WHY1相互作用并促进其与叶绿体DNA的结合。cnd1突变体的核细胞周期进程和光合作用的缺陷分别被区室限制性CND 1定位所拯救。光促进CND1与HSP90的结合及其向叶绿体的输入。本研究为跨细胞器的基因组状态趋同协调调控细胞周期以控制植物生长发育提供了一个范例。
The coordination of chloroplast and nuclear genome status is critical for plant cell function. Here, we report thatArabidopsisCHLOROPLAST AND NUCLEUS DUAL-LOCALIZED PROTEIN 1 (CND1) maintains genome stability in the chloroplast and the nucleus. CND1 localizes to both compartments, and complete loss of CND1 results in embryo lethality. Partial loss of CND1 disturbs nuclear cell-cycle progression and photosynthetic activity. CND1 binds to nuclear pre-replication complexes and DNA replication origins and regulates nuclear genome stability. In chloroplasts, CND1 interacts with and facilitates binding of the regulator of chloroplast genome stability WHY1 to chloroplast DNA. The defects in nuclear cell-cycle progression and photosynthesis ofcnd1mutants are respectively rescued by compartment-restricted CND1 localization. Light promotes the association of CND1 with HSP90 and its import into chloroplasts. This study provides a paradigm of the convergence of genome status across organelles to coordinately regulate cell cycle to control plant growth and development.