Requirement for two copies of RNA polymerase α subunit C-terminal domain for synergistic transcription activation at complex bacterial promoters
Requirement for two copies of RNA polymerase α subunit C-terminal domain for synergistic transcription activation at complex bacterial promoters
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DOI：
10.1101/gad.237502
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发表时间：
2002-10-01
影响因子：
10.5
通讯作者：
Busby, SJW
中科院分区：
文献类型：
--
作者：
Lloyd, GS;Niu, W;Busby, SJW
Transcription activation by the Escherichia coli cyclic AMP receptor protein (CRP) at different promoters has been studied using RNA polymerase holoenzyme derivatives containing two full-length et subunits, or containing one full-length alpha subunit and one truncated alpha subunit lacking the alpha C-terminal domain (alphaCTD). At a promoter having a single DNA site for CRP, activation requires only one full-length ut subunit. Likewise, at a promoter having a single DNA site for CRP and one adjacent UP-element subsite (high-affinity DNA site for alphaCTD), activation requires only one full-length at subunit. In contrast, at promoters having two DNA sites for CRP, or one DNA site for CRP and two UP-element subsites, activation requires two full-length (alpha subunits. We conclude that a single copy of alphaCTD is sufficient to interact with one CRP molecule and one adjacent UP-element subsite, but two copies of aCTD are required to interact with two CRP molecules or with one CRP molecule and two UP-element subsites.
