The analysis of vitamin C concentration in organs of gulo(-/-) mice upon vitamin C withdrawal.
The analysis of vitamin C concentration in organs of gulo(-/-) mice upon vitamin C withdrawal.
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DOI：
10.4110/in.2012.12.1.18
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发表时间：
2012-02
期刊：
影响因子：
6
通讯作者：
Lee WJ
中科院分区：
文献类型：
--
作者：
Kim H;Bae S;Yu Y;Kim Y;Kim HR;Hwang YI;Kang JS;Lee WJ
维生素C是维持人类生命所必需的营养物质。血浆中维生素C不足与坏疽的发生密切相关。然而，维生素C在体内的储存和消耗动力学仍然很不清楚。我们使用的是Gulo-/-小鼠，它不能像人类那样合成维生素C。5周后检测小鼠血浆和脏器中维生素C水平，并与野生型小鼠进行比较。在停用维生素C后3周，Gulo-/-小鼠的体重显著下降。然而，野生型和补充维生素C的古洛/-鼠之间没有差异(饮用水中3.3g/L)。停用维生素C后1周，血浆和脏器中维生素C浓度显著降低。维生素C优先沉积在肾上腺、淋巴结、肺和脑中。停用维生素C 4周后，Gulo-/-小鼠脾细胞数量和CD_4/CD_8比值无明显变化。在停用维生素C后5周，Gulo-/-小鼠的脾结构被破坏。从停用维生素C后1周开始，Gulo-/-小鼠的维生素C水平显著下降。维生素C优先储存在大脑、肾上腺和肺等器官中。
Vitamin C is an essential nutrient for maintaining human life. Vitamin C insufficiency in the plasma is closely related with the development of scurvy. However, in vivo kinetics of vitamin C regarding its storage and consumption is still largely unknown. We used Gulo-/- mice, which cannot synthesize vitamin C like human. Vitamin C level in plasma and organs from Gulo-/- mice was examined, and it compared with the level of wild-type mice during 5 weeks. The significant weight loss of Gulo-/- mice was shown at 3 weeks after vitamin C withdrawal. However, there was no differences between wild-type and vitamin C-supplemented Gulo-/- mice (3.3 g/L in drinking water). The concentration of vitamin C in plasma and organs was significantly decreased at 1 week after vitamin C withdrawal. Vitamin C is preferentially deposited in adrenal gland, lymph node, lung, and brain. There were no significant changes in the numbers and CD4/CD8 ratio of splenocytes in Gulo-/- mice with vitamin C withdrawal for 4 weeks. And the architecture of spleen in Gulo-/- mice was disrupted at 5 weeks after vitamin C withdrawal. The vitamin C level of Gulo-/- mice was considerably decreased from 1 week after vitamin C withdrawal. Vitamin C is preferentially stored in some organs such as brain, adrenal gland and lung.