Electrospray mass spectrometry of poly(propylene imine) dendrimers - The issue of dendritic purity or polydispersity
Electrospray mass spectrometry of poly(propylene imine) dendrimers - The issue of dendritic purity or polydispersity
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DOI：
10.1002/chem.19970030916
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发表时间：
1997-09-01
影响因子：
4.3
通讯作者：
Meijer, EW
中科院分区：
文献类型：
--
作者：
Hummelen, JC;vanDongen, JLJ;Meijer, EW
用电喷雾-质谱法详细分析了前五代含4-端基的聚丙烯亚胺树枝状大分子的结构，尽管制备这些树枝状大分子的反应顺序得到了高度优化，但在较高的几代中观察到了统计缺陷的积累。一个简单的统计模拟可以指定去卷积光谱中的所有峰，并确定每一代形成中每一副反应的产率。第五代含胺端基的聚丙烯亚胺树枝状大分子是经过248个连续反应步骤形成的，其树枝状纯度约为。20%，相当于约1.002的多分散度。将树枝状大分子发散生长模式中的缺陷与Merrifield多肽合成中观察到的缺陷进行了比较。
Electrospray mass spectrometry (ESI-MS) is used to analyze in detail the structure of the first five generations of the poly(propylene imine) dendrimers with 4-64 end groups, Although the reaction sequence to prepare these dendrimers is highly optimized, an accumulation of statistical defects is observed at higher generations. A simple statistical simulation allows the assignment of all peaks in deconvoluted spectrum and the determination of yields for each side reaction in the formation of each generation. The fifth generation of the poly(propylene imine) dendrimer containing amine end groups, which is formed after 248 consecutive reaction steps, possesses a dendritic purity of approx. 20%, which corresponds to a polydispersity of approximately 1.002. The defects in the divergent growth patterns of dendrimers are compared to those observed in the Merrifield synthesis of polypeptides.