INITIAL TRACT FORMATION IN THE BRAIN OF THE CHICK-EMBRYO - SELECTIVE EXPRESSION OF THE BEN/SC1/DM-GRASP CELL-ADHESION MOLECULE
INITIAL TRACT FORMATION IN THE BRAIN OF THE CHICK-EMBRYO - SELECTIVE EXPRESSION OF THE BEN/SC1/DM-GRASP CELL-ADHESION MOLECULE
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DOI：
10.1111/j.1460-9568.1995.tb01056.x
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发表时间：
1995-02-01
影响因子：
3.4
通讯作者：
SOTELO, C
中科院分区：
文献类型：
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作者：
CHEDOTAL, A;POURQUIE, O;SOTELO, C
本研究报告了鸡胚前脑和中脑第一轴突束形成早期阶段BEN表达（免疫球蛋白超家族的一种分子）的时空模式[Hamburger和汉密尔顿（HH）阶段12-22]。BEN的表达已经用免疫组织化学和非放射性原位杂交进行了分析。此外，双标记实验（结合抗III类β-微管蛋白，泛神经元标记物，和抗BEN抗体）已进行，以确定是否BEN表达的所有第一轴突束。第一个表达BEN的神经元出现在HH 13 -14期左右，位于间脑尾部。它们属于Cajal间质核，其轴突是内侧纵束的第一组成部分。到HH 14，另外两个早期轴突束出现：后连合束和三叉神经中脑核的降根。在发育后期，端脑、间脑和中脑区出现大量新的轴突束，只有少数轴突束（第四神经、外侧丘系、顶盖延髓束和缰脚束）表达BEN。在所有BEN阳性系统中，细胞体、轴突和生长锥均被抗体标记。我们已经发现，没有早期轴突束生长优先在interneuromeric边界。此外，每个束由几个薄的束而不是一个单独的束形成。BEN的表达是短暂的，在孵化前不久消失。这些结果表明，BEN可能有助于促进参与“轴突支架”的精确神经元系统的轴突生长。
This study reports the spatio-temporal pattern of BEN expression (a molecule of the immunoglobulin superfamily) during early stages of the first axonal tract formation, in the fore- and midbrain of chick embryos [Hamburger and Hamilton (HH) stages 12-22]. The expression of BEN has been analysed using immunohistochemistry and non-radioactive in situ hybridization. Furthermore, double labelling experiments (combining anti-class III beta-tubulin, a pan-neuronal marker, and anti-BEN antibodies) have been carried out to determine whether BEN is expressed by all first axonal tracts. The first neurons expressing BEN appear around stage HH13-14, in the caudal diencephalon. They belong to the interstitial nucleus of Cajal, and their axons are the first components of the medial longitudinal fasciculus. By HH14, two other early axonal tracts appear: the tract of the postoptic commissure and the descending root of the mesencephalic nucleus of the trigeminal nerve. Only the latter expresses BEN, At later stages of development numerous new axonal tracts appear in the telencephalic, diencephalic and mesencephalic domains, Only a few of them (the fourth nerve, the lemniscus lateralis, the tectobulbar and habenulopeduncular tracts) express BEN. In all BEN positive systems, the cell bodies, axons and growth cones are uniformly labelled by the antibody. We have found that none of the early axonal tracts grows preferentially at interneuromeric boundaries. Moreover, each tract is formed by several thin fascicles rather than a single one. The expression of BEN is transient and disappears shortly before hatching. These results suggest that BEN may serve to promote axonal outgrowth of precise neuronal systems involved in 'axonal scaffolding'.