Expression of H type 1 antigen of ABO histo-blood group in normal colon and aberrant expressions of H type 2 and H type 3/4 antigens in colon cancer
Expression of H type 1 antigen of ABO histo-blood group in normal colon and aberrant expressions of H type 2 and H type 3/4 antigens in colon cancer
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DOI：
10.1023/a:1007173722426
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发表时间：
2000-05-01
影响因子：
3
通讯作者：
Kimura, H
中科院分区：
文献类型：
--
作者：
Fujitani, N;Liu, Y;Kimura, H
我们用三种针对H型1/2、H型2和H型3/4链的单抗，用免疫组织化学方法检测了ABO(H)组织血型系统的1型、2型和3/4型链在人正常结肠和结肠癌中的分布。我们意外地发现，来自分泌者的正常结肠粘膜仅表达H型1，而不表达H型3/4。H型1表达于杯状细胞。表达H-1型的杯状细胞数量从近端结肠到直肠逐渐减少。在肿瘤中，7例分泌性近端结肠癌组织中有4例(57%)未表达H型1，而8例分泌性远端结肠癌组织中均有H1表达。近端和远端结肠癌组织中均有H2型和H型3/4的异常表达(分别为47%和67%)。非分泌型肿瘤不表达任何H抗原。我们的结果表明，正常结肠组织中H型1的表达以及结肠癌组织中H2型和H型3/4的异常表达受FUT2编码的s型α(1，2)岩藻糖基转移酶的调控。然而，UEA-I阳性物质(S)而不是H2型在正常结肠和结肠癌中唯一表达，无论是分泌型还是非分泌型。
We have immunohistochemically examined the distribution of the H antigens of type 1, type 2 and type 3/4 chains of the ABO(H) histo-blood group system in human normal colon and in colon cancer using three monoclonal antibodies specific for each of the H type 1/2, H type 2, and the H type 3/4 chain. We unexpectedly found that mucosa of the normal colon from secretors but not that from nonsecretors expressed only H type 1 and did not express H type 2 or H type 3/4. The H type 1 was expressed in goblet cells. Positive goblet cells expressing H type 1 were decreased in number progressively from the proximal colon to the rectum. In tumors, 4 (57%) of 7 cancer tissues of the proximal colon from secretors expressed no H type 1, whereas all 8 cancer tissues of the distal colon from secretors expressed H type 1. The aberrant expressions of H type 2 and H type 3/4 (47 and 67%, respectively) were found in cancer tissues from both the proximal and the distal colon. Tumors from nonsecretors did not express any H antigens. Our results suggested that the expression of H type 1 in the normal colon and the aberrant expressions of H type 2 and H type 3/4 in colon cancer tissues were regulated by FUT2-encoded Se type alpha (1,2)fucosyltransferase. However, UEA-I-positive substance(s) rather than H type 2 were uniquely expressed throughout the normal colon and in colon cancers from both secretors and nonsecretors.