Sex differences in oestrogen-induced p44/42 MAPK phosphorylation in the mouse brain in vivo
Sex differences in oestrogen-induced p44/42 MAPK phosphorylation in the mouse brain in vivo
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DOI：
10.1111/j.1365-2826.2006.01447.x
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发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Abraham, I. M.
中科院分区：
文献类型：
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作者：
Barabas, K.;Szego, E. M.;Abraham, I. M.
除了经典的直接基因组作用机制外，雌激素还对神经元的信号系统产生知之甚少的非经典效应。在本研究中，我们调查是否存在性别差异，在性腺切除术和雌激素诱导的p44/42丝裂原活化蛋白激酶（MAPK）磷酸化在特定的大脑区域的小鼠。我们证明，MAPK免疫反应性没有改变性腺切除术或雌激素治疗的任何性别。然而，我们发现，磷酸化MAPK（pMAPK）的前腹侧室周核（AVPV）内的水平始终高于男性比女性，无论性腺类固醇激素的状态。此外，性腺切除术被发现减少pMAPK免疫反应性的男性梨状皮质内。雌激素增加pMAPK的免疫反应性在内侧视前区和AVPV的女性，但没有在雄性小鼠具有相同的效果。总之，这些结果表明，雌激素诱导的体内脑内MAPK磷酸化改变存在显著的性别差异。
In addition to the classical direct genomic mechanisms of action, oestrogen also exerts poorly understood, nonclassical effects on the signalling system in neurones. In the present study, we investigated whether sex differences exist in gonadectomy- and oestrogen-induced effects on p44/42 mitogen-activated protein kinase (MAPK) phosphorylation in specific brain regions of mice. We demonstrate that MAPK immunoreactivity was not altered by gonadectomy or oestrogen treatment in either sex. However, we show that the level of phosphorylated MAPK (pMAPK) within the anteroventral periventricular nucleus (AVPV) was consistently higher in males than females irrespective of gonadal steroid hormone status. In addition, gonadectomy was found to decrease pMAPK immunoreactivity within the piriform cortex of males. Oestrogen increased pMAPK immunoreactivity in the medial preoptic area and AVPV of females, but failed to have the same effect in male mice. Overall, these results demonstrate a marked sex difference in oestrogen-induced alteration of MAPK phosphorylation in the brain in vivo.