MICROVILLI AND BLEBS AS SOURCES OF RESERVE SURFACE-MEMBRANE DURING CELL SPREADING
MICROVILLI AND BLEBS AS SOURCES OF RESERVE SURFACE-MEMBRANE DURING CELL SPREADING
复制标题
DOI：
10.1016/0014-4827(76)90595-4
复制
发表时间：
1976-01-01
影响因子：
3.7
通讯作者：
TRINKAUS, JP
中科院分区：
文献类型：
--
作者：
ERICKSON, CA;TRINKAUS, JP
在扫描EM中，在同步的[幼仓鼠肾] BHK 21细胞中检查了细胞从圆形扩展到扁平状态时发生的表面积增加。圆形的细胞，无论是处于有丝分裂还是分离并新鲜接种在培养物中，都被褶皱、水泡和微绒毛的混合物覆盖。随着细胞的扩散，这些突起消失，首先在扁平的边缘区域，然后逐渐消失，直到整个细胞表面几乎光滑。圆形的有丝分裂后的子细胞，考虑到微绒毛的估计表面积，接近有丝分裂后4小时完全展开的细胞。同样，圆形的早期有丝分裂母细胞也被微绒毛覆盖，其表面与有丝分裂前完全展开的细胞大致相同。细胞在其微绒毛和其他突起中显然具有膜储备，可用于扩展和启动细胞运动。
The increase in surface area that occurs as cells spread from the rounded to the flattened state was examined in synchronized [baby hamster kidney] BHK 21 cells in the scanning EM. Rounded cells, whether in mitosis or dissociated and freshly seeded in culture, are covered with a mixture of folds, blebs, and microvilli. As cells spread, these protuberances disappear, first in the flattening marginal region and pregressively submarginally until the entire cell surface is virtually smooth. The estimated surface area of rounded post-mitotic daughter cells, taking microvilli into account, is close to that of fully spread cells 4 h after mitosis. Likewise, rounded early mitotic mother cells, which are also covered with microvilli, have approximately the same surface as fully spread cells just prior to mitosis. Cells apparently possess a membrane reserve in their microvilli and other protuberances which can be utilized for spreading and initiating cell locomotion.