SOME METABOLIC ASPECTS OF TOLERANCE TO BACTERIAL ENDOTOXIN
SOME METABOLIC ASPECTS OF TOLERANCE TO BACTERIAL ENDOTOXIN
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DOI：
10.1128/jb.90.4.970-977.1965
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发表时间：
1965-01-01
影响因子：
3.2
通讯作者：
SMYTHE, DS
中科院分区：
文献类型：
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作者：
BERRY, LJ;SMYTHE, DS
当放线菌素D与热杀死的细胞一起注射时，小鼠对热杀死的鼠伤寒沙门氏菌产生了对细菌内毒素的耐受性。当与内毒素一起给药时，乙硫氨酸和2-硫脲嘧啶均未改变耐受性的发展。注射内毒素，放线菌素D或蛋氨酸在注射后4或17小时显著降低正常小鼠肝酶色氨酸吡啶酶的活性。同样，注射糖化氧化铁或Thorotrast降低肝脏色氨酸吡咯酶活性，在正常动物中比在耐受动物中更广泛。观察到耐受小鼠网状内皮系统（RES）的激活，通过血液中碳清除速度加快来确定，但这是通过适当剂量的放线菌素d来阻止的。当糖化氧化铁而不是内毒素时获得类似的结果，并且在注射内毒素后，它们的色氨酸吡啶酶通常会减少。因此，RES激活可能在耐受性未形成的情况下发生。更接近正常水平的酶活性似乎是耐药状态的特征。
The tolerance to bacterial endotoxins which is produced in mice given a series of daily injections of heat-killed Salmonella typhimurium failed to occur when actinomycin D was administered with the heat-killed cells. Neither ethionine nor 2-thiouracil, when given with endotoxin, altered the development of tolerance. As injection of endotoxin, actinomycin D, or ethionine lowered the activity of the liver enzyme tryptophan pyrrolase more significantly at either 4 or 17 hr postinjection in normal mice than in tolerant mice. Similarly, an injection of either saccharated iron oxide or Thorotrast lowered liver tryptophan pyrrolase activity more extensively in normal than in tolerant animals. Activation of the reticuloendothelial system (RES) of tolerant mice, as determined by an accelerated rate of carbon clearance from the blood, was observed, but this was prevented by the appropriate dose of actinomycin D. Similar results were obtained when saccharated iron oxide, rather than endotoxin and their tryptophan pyrrolase was normally diminished after an injection of endotoxin. Thus, RES activation may occur without tolerance developing. A more nearly normal level of enzyme activity appears to be characteristic of the tolerant state.