Antifungal metabolites from Phomopsis sp. By254, an endophytic fungus in Gossypium hirsutum
Antifungal metabolites from Phomopsis sp. By254, an endophytic fungus in Gossypium hirsutum
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DOI：
10.5897/ajmr11.272
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发表时间：
2011-05
影响因子：
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通讯作者：
Fu Jing;Zhou Yang;L. Feng;Y. Hao;G. Hua
中科院分区：
文献类型：
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作者：
Fu Jing;Zhou Yang;L. Feng;Y. Hao;G. Hua
对拟茎点霉的氯仿-甲醇 (1:1) 提取物进行化学和生物测定指导的分级分离。 By254，一种存在于陆地棉根部的内生真菌，产生了三种化合物。通过结合光谱分析（MS、1D 和 2D-NMR）将这些化合物的结构阐明为环氧细胞松弛素 H (1)、细胞松弛素 N (2) 和细胞松弛素 H (3)。所有化合物均对植物病原真菌和植物病原细菌具有抗菌作用。结果，化合物1至3显着抑制核盘菌、玉米双极菌、尖孢镰刀菌、灰葡萄孢、索罗基尼双极菌、禾本科全脂酵母的生长。小麦和谷类丝核菌，IC50 值范围为 0.1 至 50 g/ml。然而，没有观察到它们对植物病原菌有抑制作用。
Chemical and bioassay-guided fractionation of the chloroform-methanol (1:1) extract of Phomopsis sp. By254, an endophytic fungus residing in the root of Gossypium hirsutum, yielded three compounds. The structures of these compounds were elucidated by a combination of spectroscopic analysis (MS, 1Dand 2D-NMR) to be epoxycytochalasin H (1), cytochalasin N (2), and cytochalasin H (3). All the compounds were subjected to antimicrobial action against plant pathogenic fungi and plant pathogenic bacteria. As a result, compounds 1 to 3 significantly inhibited the growth of Sclerotinia sclerotiorum, Bipolaris maydis, Fusarium oxysporum, Botrytis cinerea, Bipolaris sorokiniana, Gaeumannomyces graminis var. tritici and Rhizoctonia cerealis, with IC50 value ranging from 0.1 to 50 g/ml. However, no inhibition of them was observed against plant pathogenic bacteria.