Three-dimensional structure of a DNA hairpin in solution: two-dimensional NMR studies and distance geometry calculations on d(CGCGTTTTCGCG).
Three-dimensional structure of a DNA hairpin in solution: two-dimensional NMR studies and distance geometry calculations on d(CGCGTTTTCGCG).
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溶液中 DNA 发夹的三维结构：二维 NMR 研究和 d(CGCGTTTTCGCG) 的距离几何计算。
DOI：
10.1021/bi00366a053
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Reid,BR
中科院分区：
文献类型：
--
作者：
Hare,DR;Reid,BR
材料和方法DNA十二聚体d(CGTTTTCGCG)是用固相亚磷酸三酯技术合成的(Hare等人，1983；Chou等人，1984)，并溶解在含有10 mM磷酸二氢钠的0.4毫升缓冲液中，pH 7.0。样品冻干至干燥，加入99.996%D20 0.4毫升。再来一次之后
Materials and MethodsThe DNA dodecamer d (CGCGTTTTCGCG) was synthesized by using solid-phase phosphite triester techniques as described previously (Hare et al., 1983; Chou et al., 1984) and was dissolved in 0.4 mL of a buffer containing 10 mM sodium phosphate, pH 7.0. The sample was lyophilized to dryness, and 0.4 mL of 99.996% D20 was added. After one more