Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis
Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis
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DOI：
10.1105/tpc.104.022830
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发表时间：
2004-08-01
期刊：
影响因子：
11.6
通讯作者：
Zhu, JK
中科院分区：
文献类型：
--
作者：
Sunkar, R;Zhu, JK
MicroRNAs(MiRNAs)和Short Interfering RNAs(SiRNAs)是近年来出现的非编码小RNA，是mRNA降解、翻译抑制和染色质修饰的重要调节因子。在拟南芥中，迄今已报道了15个家族的43个miRNAs。为了鉴定新的和非生物胁迫调节的miRNAs和siRNAs，我们构建了一个从受到脱水、盐分、冷胁迫或植物胁迫激素脱落酸胁迫的拟南芥幼苗中获得的小RNA文库。对文库进行测序和随后的分析，从34个基因座发现了26个新的miRNAs，形成了15个新的家族。来自三个基因座的两个新miRNAs是先前报道的miR171和miR319家族的成员。一些miRNAs在特定的组织中优先表达，还有一些在非生物胁迫下表达上调或下调。其中10个miRNAs在其他植物中高度保守。根据序列互补性，对新发现的miRNAs预测了51个具有不同功能的潜在靶点。除了miRNAs，我们还在拟南芥中发现了102个新的内源小RNA。这些发现表明，拟南芥基因组编码了大量的miRNAs和其他小的调控RNA，其中一些可能在植物对环境胁迫的反应以及在发育和基因组维护中发挥重要作用。
MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are small noncoding RNAs that have recently emerged as important regulators of mRNA degradation, translational repression, and chromatin modification. In Arabidopsis thaliana, 43 miRNAs comprising 15 families have been reported thus far. In an attempt to identify novel and abiotic stress regulated miRNAs and siRNAs, we constructed a library of small RNAs from Arabidopsis seedlings exposed to dehydration, salinity, or cold stress or to the plant stress hormone abscisic acid. Sequencing of the library and subsequent analysis revealed 26 new miRNAs from 34 loci, forming 15 new families. Two of the new miRNAs from three loci are members of previously reported miR171 and miR319 families. Some of the miRNAs are preferentially expressed in specific tissues, and several are either upregulated or downregulated by abiotic stresses. Ten of the miRNAs are highly conserved in other plant species. Fifty-one potential targets with diverse function were predicted for the newly identified miRNAs based on sequence complementarity. In addition to miRNAs, we identified 102 other novel endogenous small RNAs in Arabidopsis. These findings suggest that a large number of miRNAs and other small regulatory RNAs are encoded by the Arabidopsis genome and that some of them may play important roles in plant responses to environmental stresses as well as in development and genome maintenance.